Highly efficient CRISPR-Cas9 base editing in Bifidobacterium with bypass of restriction modification systems.
Lin H-C, Hsiao W-C, Hsu Y-C, Lin M-C, Hsu C-C, Zhang MM
Appl Environ Microbiol. 2025 Mar 10:e0198524. doi: 10.1128/aem.01985-24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234660 | cBEST4 | Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion |