A novel expression system for imaging single-molecule fluorescence in Haloferax volcanii WR806 enables visualization of altered Cas1 dynamics during UV-induced DNA damage response
Schrage PR, Afonina U, Wörtz J, Marchfelder A, Martens KJA, Sáenz JP, Endesfelder U
μLife
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234669 | pUE001-Dendra2Hfx | encodes Dendra2Hfx in Haloferax volcanii under the tryptophan inducible p.tna promoter, trpA, ampR, shuttle vector for E.coli |
| 234670 | pUE001-Cas1:Dendra2Hfx | encodes Cas1:Dendra2Hfx fusion in Haloferax volcanii under the tryptophan inducible p.tna promoter, trpA, ampR, shuttle vector for E.coli |
| 234671 | pUE001-FtsZ1:Dendra2Hfx | encodes FtsZ1:Dendra2Hfx fusion in Haloferax volcanii under the tryptophan inducible p.tna promoter, trpA, ampR, shuttle vector for E.coli |