Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase
Witte IP, Lampe GD, Eitzinger S, Miller SM, Berríos KN, McElroy AN, King RT, Stringham OG, Gelsinger DR, Vo PLH, Chen AT, Tolar J, Osborn MJ, Sternberg SH, Liu DR
Science 388, eadt5199(2025).
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234725 | pCMV-evoCAST-Cas6 | Engineered human-codon optimized PseCas6 in mammalian cells (for evoCAST) |
| 234726 | pCMV-evoCAST-eCas7 | Engineered human-codon optimized PseCas7 in mammalian cells (for evoCAST) |
| 234727 | pCMV-evoCAST-eCas8 | Engineered human-codon optimized PseCas8 in mammalian cells (for evoCAST) |
| 234728 | pCMV-evoCAST-TniQ | Engineered human-codon optimized PseTniQ in mammalian cells (for evoCAST) |
| 234729 | pCMV-evoCAST-evoTnsAB | Evolved human-codon optimized PseTnsAB in mammalian cells (for evoCAST) |
| 234730 | pCMV-evoCAST-evoTnsC | Evolved human-codon optimized PseTnsC in mammalian cells (for evoCAST) |
| 234731 | pCMV-wtTnsAB | Wild-type human-codon optimized PseTnsAB in mammalian cells |
| 234732 | pCMV-wtTnsC | Wild-type human-codon optimized PseTnsC in mammalian cells |
| 234733 | pCMV-ClpX | Human-codon optimized E. coli ClpX in mammalian cells |
| 234734 | pCMV-PuroR-T2A-eGFP | Puromycin resistance/eGFP cassette in mammalian cells (transfection marker) |
| 234735 | pU6-AAVS1-5-1kbdonor | PseCAST crRNA targeting AAVS1-5, with 1 kb donor transposon |
| 234736 | pU6-hROSA26-1-1kbdonor | PseCAST crRNA targeting hROSA26-1, with 1 kb donor transposon |