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NGN3 oscillatory expression controls the timing of human pancreatic endocrine differentiation.

Miller A, Biga V, Rowntree A, Chhatriwala M, Woods F, Noble B, Marinopoulou E, Kamath A, Vallier L, Papalopulu N
Dev Cell. 2025 Sep 22;60(18):2518-2532.e9. doi: 10.1016/j.devcel.2025.06.004. Epub 2025 Jun 25. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
234887CAG-NGN3-linker-3xFLAG-Dendra2-HAExpresses human NGN3 in mammalian cells directly fused to Dendra2 fluorescent protin plus FLAG and HA tags
234888CAG-PM-NGN3-linker-3xFLAG-Dendra2-HAExpresses human phosphomutant-NGN3 in mammalian cells directly fused to Dendra2 fluorescent protin plus FLAG and HA tags
234890CAG-NLS-mScarleti-NLS-IRES-PuromycinExpressed a nuclear mScarleti fluorophoro in mammalian cells

Antibodies from Article