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Oscar Ramos Lab: Ramos SBE lab plasmids

Unpublished

Plasmids from Article

ID Plasmid Purpose
235097VN519 pBR-rpoA-IP3mut3AQuantitative bacterial two-hybrid (qB2H). Expresses IP3mut3A.
235098VN515 pBR-rpoA-IP1Quantitative bacterial two-hybrid (qB2H). Expresses IP1.
235099VN516 pBR-rpoA-IP2Quantitative bacterial two-hybrid (qB2H). Expresses IP2.
235100VN517 pBR-rpoA-IP3Quantitative bacterial two-hybrid (qB2H). Expresses IP3.
235101VN518 pBR-rpoA-IP4Quantitative bacterial two-hybrid (qB2H). Expresses IP4.
235102VN550 pAC-cI_E34P-Asf1A_Term_f1-pOL2-62_L-eGFP-KanRQuantitative bacterial two-hybrid (qB2H). Expresses Asf1A.
235103VN627 pAC-cI_E34P-Asf1B_Term_f1-pOL2-62_L_eGFP-KanRQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B.
235104VN999 pB2Hws_v9_cI-Asf1B+rpoA-IP3mut3AQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3mut3A.
235105VN991 pB2Hws_v9_cI-Asf1B+rpoA-IP1Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1.
235106VN1043 pB2Hws_v9_cI-Asf1B+rpoA-IP2Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP2.
235107VN990 pB2Hws_v9_cI-Asf1B+rpoA-IP3Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3.
235108VN1335 pB2Hws_v9_cI-Asf1B+rpoA-IP4Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP4.
235109VN1312 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3mut3A_Ddcm_eGFP-wcKanRQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3mut3A.
235110VN1313 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP1_Ddcm_eGFP-wcKanRQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1.
235111VN1314 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP2_Ddcm_eGFP-wcKanRQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP2.
235112VN1315 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3_Ddcm_eGFP-wcKanRQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3.
235113VN1316 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP4_Ddcm_eGFP-wcKanRQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP4.
235114VN1264 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3mut3A_Ddcm_WeaKRBS_TIR51_KanRQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3mut3A.
235115VN1263 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP1_Ddcm_WeaKRBS_TIR51_KanRQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1.
235116VN1287 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP2_Ddcm_WeaKRBS_TIR51_KanRQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP2.
235117VN1262 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3_Ddcm_WeaKRBS_TIR51_KanRQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3.
235118VN1852 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP4_Ddcm_WeaKRBS_TIR51_KanRQuantitative bacterial two-hybrid (qB2H). Expresses ASf1B and IP4.
235119VN1897 pB2Hws_v28_Ddcm_wkKanR_TIR51_pLWeakRBS_TIR371_cI-Asf1B+rpoA-2GGQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B. In fusion cloning to rpoA using BsaI based golden gate.
235120VN1272 pB2Hws_v28_Ddcm_wkKanR_TIR51_pLWeakRBS_TIR371_cI-2GG+rpoA-IP3Quantitative bacterial two-hybrid (qB2H). Expresses IP3.

Antibodies from Article