MicroRNA mechanisms instructing Purkinje cell specification.
Zolboot N, Xiao Y, Du JX, Ghanem MM, Choi SY, Junn MJ, Zampa F, Huang Z, MacRae IJ, Lippi G
Neuron. 2025 Apr 1:S0896-6273(25)00181-3. doi: 10.1016/j.neuron.2025.03.009.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235139 | pAAV-hSyn-FLAG-HA-T6B-WT-YFP | Expresses the T6B peptide fused with YFP under the human synapsin promoter |
| 235140 | pAAV-hSyn-FLAG-HA-T6B-MUT-YFP | Expresses the mutant T6B peptide fused with YFP under the human synapsin promoter |
| 235141 | pAAV-L7-6-FLAG-HA-T6B-WT-YFP | Expresses the T6B peptide fused with YFP under the Purkinje cell-specific L7-6 promoter |
| 235142 | pAAV-L7-6-FLAG-HA-T6B-MUT-YFP | Expresses the mutant T6B peptide fused with YFP under the Purkinje cell-specific L7-6 promoter |
| 235143 | pAAV-hSyn-DD-T6B-WT-YFP | Expresses the inducible T6B peptide fused with DHFR and YFP under the human synapsin promoter |
| 235144 | pAAV-hSyn-DD-T6B-MUT-YFP | Expresses the inducible mutant T6B peptide fused with DHFR and YFP under the human synapsin promoter |
| 235145 | pAAV-L7-6-DD-T6B-WT-YFP | Expresses the inducible T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoter |
| 235146 | pAAV-L7-6-DD-T6B-MUT-YFP | Expresses the inducible mutant T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoter |
| 235147 | pAAV-rev(CMV-TagBFP2)-EFS-mCherry-124-WT-MRE | Expresses the mCherry-based sensor for miR-124. TagBFP2 is expressed as a transduction reporter |
| 235148 | pAAV-rev(CMV-TagBFP2)-EFS-mCherry-124-MUT-MRE | Expresses the mCherry-based mutated (control) sensor for miR-124. TagBFP2 is expressed as a transduction reporter |
| 235149 | pET23a-His-TEV-SpyCatcher3 | Bacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site. |
| 235150 | pET23a-His-TEV-HaloTag-linker-SpyCatcher3 | Bacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site. |
| 235151 | pAAV-L7-6-Cre | Expresses Cre recombinase under the Purkinje cell-specific L7-6 promoter |
| 235152 | pAAV[FLEX]-CAG-EGFP-Ctrl-sponge | Expresses a EGFP-based control sponge in a Cre recombinase-dependent manner |
| 235154 | pAAV[FLEX]-CAG-EGFP-miR-206-sponge | Expresses a EGFP-based sponge for miR-206 in a Cre recombinase-dependent manner |
| 235155 | pAAV[FLEX]-CAG-EGFP-miR-133-sponge | Expresses a EGFP-based sponge for miR-133 in a Cre recombinase-dependent manner |
| 235156 | pAAV-rev(CMV-TagBFP2)-EFS-mCherry-206-MRE | Expresses the mCherry-based sensor for miR-206. TagBFP2 is expressed as a transduction reporter |
| 235157 | pAAV-rev(CMV-TagBFP2)-EFS-mCherry-133-MRE | Expresses the mCherry-based sensor for miR-133. TagBFP2 is expressed as a transduction reporter |
| 235158 | pAAV[FLEX]-CAG-EGFP-pri-miR-Ctrl | Expresses EGFP and a control primary transcript in a Cre recombinase-dependent manner |
| 235159 | pAAV[FLEX]-CAG-EGFP-pri-miR-137 | Expresses EGFP and miR-137 primary transcript in a Cre recombinase-dependent manner |
| 235160 | pAAV-rev(CMV-TagBFP2)-EFS-mCherry-137-MRE | Expresses the mCherry-based sensor for miR-137. TagBFP2 is expressed as a transduction reporter |