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Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system

Della Valle S, Orsi E, Creutzburg SCA, Jansen LFM, Pentari E, Beisel CL, Steel H, Nikel PI, Staals RHJ, Claassens NJ, van der Oost J, Huang WE, Patinios C
bioRxiv 2024.11.24.625072; doi: 10.1101/2024.11.24.625072 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
235167pCas12a-NTExpresses the Wild Type Francisella novicida cas12a gene and a NT crRNA
235168pSIBR2.0-818-Cas12a-NTExpresses the SIBR2.0-818-Cas12a system and a NT crRNA

Antibodies from Article