Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system.
Della Valle S, Orsi E, Creutzburg SCA, Jansen LFM, Pentari EN, Beisel CL, Steel H, Nikel PI, Staals RHJ, Claassens NJ, van der Oost J, Huang WE, Patinios C
Trends Biotechnol. 2025 Jun;43(6):1470-1491. doi: 10.1016/j.tibtech.2025.02.006. Epub 2025 Mar 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235165 | pCas9-NT | Expresses the codon harmonized Streptococcus pyogenes cas9 gene and a NT sgRNA |
| 235166 | pSIBR-Int4-Cas9-NT | Expresses the SIBR-Int4-Cas9 system and a NT sgRNA |
| 235167 | pCas12a-NT | Expresses the Wild Type Francisella novicida cas12a gene and a NT crRNA |
| 235168 | pSIBR2.0-818-Cas12a-NT | Expresses the SIBR2.0-818-Cas12a system and a NT crRNA |