Engineering intercellular communication using M13 phagemid and CRISPR-based gene regulation for multicellular computing in Escherichia coli
Kusumawardhani, H., Zoppi, F., Avendaño, R. et al.
Nat Commun 16, 3569 (2025)
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235447 | HP17deltaP8 | Helper plasmid for regulated phage transfer |
| 235449 | pHK001.1 | NOT gate receiver with binding site (bs) for sgRNA1 |
| 235450 | pHK001.2 | NOT gate receiver with bs for sgRNA2 |
| 235451 | pHK001.3 | NOT gate receiver with bs for sgRNA3 |
| 235452 | pHK001.4 | NOT gate receiver with bs for sgRNA4 |
| 235453 | pHK001.5 | NOT gate receiver with bs for sgRNA5 |
| 235454 | pHK001.6 | NOT gate receiver with bs for sgRNA6 |
| 235455 | pHK506.4 | BUF/YES gate receiver with bs for sgRNA2 |
| 235456 | pHK506.5 | BUF/YES gate receiver with bs for sgRNA3 |
| 235457 | pHK507 | OR/NAND gate receiver with bs for sgRNA2 and sgRNA3 |
| 235459 | pHK009 | NOR gate receiver with bs for sgRNA2 and sgRNA3 |
| 235460 | pHK002.1 | Message phagemid carrying sgRNA1 (prom. J23119, backbone pBR322) |
| 235461 | pHK002.2 | Message phagemid carrying sgRNA2 (prom. J23119, backbone pBR322) |
| 235462 | pHK002.3 | Message phagemid carrying sgRNA3 (prom. J23119, backbone pBR322) |
| 235463 | pHK002.4 | Message phagemid carrying sgRNA4 (prom. J23119, backbone pBR322) |
| 235464 | pHK002.5 | Message phagemid carrying sgRNA5 (prom. J23119, backbone pBR322) |
| 235465 | pHK002.6 | Message phagemid carrying sgRNA6 (prom. J23119, backbone pBR322) |
| 235466 | pHK302.1 | Message phagemid carrying sgRNA1 (prom. J23110, backbone pBR322) |
| 235467 | pHK302.2 | Message phagemid carrying sgRNA2 (prom. J23110, backbone pBR322) |
| 235468 | pHK302.3 | Message phagemid carrying sgRNA3 (prom. J23110, backbone pBR322) |
| 235469 | pHK302.4 | Message phagemid carrying sgRNA4 (prom. J23110, backbone pBR322) |
| 235470 | pHK302.5 | Message phagemid carrying sgRNA5 (prom. J23110, backbone pBR322) |
| 235471 | pHK302.6 | Message phagemid carrying sgRNA6 (prom. J23110, backbone pBR322) |
| 235472 | pHK052.3 | Message phagemid carrying sgRNA3 (prom. J23119, backbone RSF1030) |
| 235473 | pHK352.3 | Message phagemid carrying sgRNA3 (prom. J23110, backbone RSF1030) |
| 235474 | pHK316 | Inducible gene VIII by Ptet promoter |
| 235475 | pHK326 | Inducible gene VIII by Ptac promoter |
| 235476 | pHK336 | Inducible gene VIII by Plux promoter |
| 235477 | pHK346 | Inducible gene VIII by Pbad promoter |
| 235478 | pHK318 | Inducible gene VIII by Plux/tet hybrid promoter |
| 235479 | pHK328 | Inducible gene VIII by Pbad/lac hybrid promoter |
| 235481 | pHK201 | Repression of gene VIII by Ptac promoter |
| 235482 | pHK401 | Repression of gene VIII by Plux/tet hybrid promoter |
| 235484 | pHK001.23 | dual NOT gate receiver with bs for sgRNA2 and sgRNA3 |
| 235485 | pHK001.3QS | inducible NOT gate receiver with bs for sgRNA3 |
| 235486 | pHK-cinI | Inducible gene cinI for OC14-HSL production by Ptac promoter |