Systematic epigenome editing captures the context-dependent instructive function of chromatin modifications.
Policarpi C, Munafo M, Tsagkris S, Carlini V, Hackett JA
Nat Genet. 2024 Jun;56(6):1168-1180. doi: 10.1038/s41588-024-01706-w. Epub 2024 May 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235571 | pPB_mU6_enh-gRNA_Puro-T2A-BFP | Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites. |
| 235572 | pPB_TRE3G_dCas9-5xGCN4_Hygro | Dox-inducible expression of dCas9-GCN4 & genomic integration |
| 235573 | pPB_TRE3G_scFV-sfGFP | Dox-inducible expression of control superfolder (sf)GFP fused with scFV |
| 235574 | pPB_TRE3G_scFV-sfGFP-Ring1bCD | Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Ring1b fused with scFV to programme H2AK119ub. |
| 235575 | pPB_TRE3G_scFV-sfGFP-Ezh2FL | Dox-inducible expression of epigenetic effector Ezh2 fused with scFV to programme H3K27me3 |
| 235576 | pPB_TRE3G_scFV-sfGFP-Dnmt3a3lCD | Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dnmt3a3L fused with scFV to programme DNA methylation |
| 235577 | pPB_TRE3G_scFV-sfGFP-Prdm9CD | Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3 |
| 235579 | pPB_TRE3G_scFV-sfGFP-Setd2CD | Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Setd2 fused with scFV to programme H3K36me3 |
| 235580 | pPB_TRE3G_scFV-sfGFP-G9aCD | Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector G9a fused with scFV to programme H3K9me2/3 |
| 235581 | pPB_TRE3G_scFV-sfGFP-Kmt5cFL | Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Kmt5c fused with scFV to programme H4K20me3 |
| 235582 | pPB_TRE3G_scFV-sfGFP-Dot1LCD | Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dot1l fused with scFV to programme H3K79me2 |
| 235583 | pPB_TRE3G_scFV-sfGFP-Ring1bCD-CatMut | Dox-inducible expression of control catalytic-mutant Ring1b CD fused with scFV |
| 235584 | pPB_TRE3G_scFV-sfGFP-Ezh2FL-CatMut | Dox-inducible expression of control catalytic-mutant Ezh2 fused with scFV |
| 235586 | pPB_TRE3G_scFV-sfGFP-Prdm9CD-CatMut | Dox-inducible expression of control catalytic-mutant Prdm9 CD fused with scFV |
| 235588 | pPB_TRE3G_scFV-sfGFP-Setd2CD-CatMut | Dox-inducible expression of control catalytic-mutant Setd2 CD fused with scFV |
| 235590 | pPB_TRE3G_scFV-sfGFP-Kmt5cFL-CatMut | Dox-inducible expression of control catalytic-mutant Kmt5c CD fused with scFV |
| 235591 | pPB_TRE3G_scFV-sfGFP-Dot1LCD-CatMut | Dox-inducible expression of control catalytic-mutant Dot1l CD fused with scFV |