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Secondary interactions in ubiquitin-binding domains achieve linkage or substrate specificity.

Michel MA, Scutts S, Komander D
Cell Rep. 2024 Aug 27;43(8):114545. doi: 10.1016/j.celrep.2024.114545. Epub 2024 Jul 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
235644pOPINK-CAPN15 NZF1 (1-42)Bacterial expression of human CAPN15 NZF1 (1-42)
235645pOPINK-CAPN15 NZF2 (34-72)Bacterial expression of human CAPN15 NZF2 (34-72)
235646pOPINK-CAPN15 (N)ZF1-3 (1-173)Bacterial expression of human CAPN15 (N)ZF1-3 (1-173)
235647pOPINK-CAPN15 ZF3 (141-173)Bacterial expression of human CAPN15 ZF3 (141-173)
235648pOPINK-CAPN15 ZF4 (337-369)Bacterial expression of human CAPN15 ZF4 (337-369)
235649pOPINK-CAPN15 ZF5 (411-444)Bacterial expression of human CAPN15 ZF5 (411-444)
235650pOPINK-HOIL-1L NZF (195-252)Bacterial expression of human HOIL-1L NZF (195-252)
235651pOPINK-Sharpin NZF (347-376)Bacterial expression of human Sharpin NZF (347-376)

Antibodies from Article