PCSK5M452I is a recessive hypomorph exclusive to MCF10DCIS.com cells.
Marohl T, Atkins KA, Wang L, Janes KA
Mol Cancer Res. 2025 Oct 28. doi: 10.1158/1541-7786.MCR-25-0211.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232445 | pDONR223 PCSK5(M452I) | Gateway pDONR vector |
| 232446 | pLX304 PCSK5(M452I)-V5 blast | Lentiviral expression vector for PCSK5(M452I) |
| 232447 | pLX304 EGFP-V5 blast | Lentiviral expression vector for EGFP |
| 232448 | pEN_TT PCSK5-V5 | Gateway entry vector for an inducible PCSK5 |
| 232449 | pEN_TT PCSK5(sgRR)-V5 | Gateway entry vector for an inducible sg09 resistant PCSK5 |
| 232450 | pEN_TT PCSK5(sgRR,M452I)-V5 | Gateway entry vector for an inducible sg09 resistant PCSK5_M452I mutant |
| 232451 | pEN_TT PCSK5(sgRR,T288P)-V5 | Gateway entry vector for an inducible sg09 resistant PCSK5_T288P mutant |
| 232452 | pSLIK PCSK5(sgRR)-V5 hygro | Lentiviral expression vector for an inducible sg09 resistant PCSK5 |
| 232453 | pSLIK PCSK5(sgRR,M452I)-V5 hygro | Lentiviral expression vector for an inducible sg09 resistant PCSK5_M452I mutant |
| 232454 | pSLIK PCSK5(sgRR,T288P)-V5 hygro | Lentiviral expression vector for an inducible sg09 resistant PCSK5_T288P mutant |
| 232455 | EDCPV PCSK5_sg09 | Lentiviral, CRISPR sgRNA for PCSK5 |
| 235669 | pLenti PGK Blast LUC (w528-1) | Lentiviral expression vector for Luciferase without v5 tag |