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Efficient plasmid-based rescue of T7 RNA polymerase-driven calicivirus reverse genetics systems in mammalian cells using vaccinia virus RNA capping enzymes

Frazer J.T. Buchanan, Markella Loi, Charlotte Chim, ShuXian Zhou, Rebekah Penrice-Randal, Leandro X. Neves, Maximillian Erdmann, Edward Emmott
bioRxiv 2026.03.19.712921; doi: 10.64898/2026.03.19.712921 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
235668pGenLenti murine CD300LF3rd Generation lentiviral vector for expression of murine CD300LF. Murine CD300LF is the receptor for murine norovirus, and expression confers susceptibility to this virus. Puromycin resistance.

Antibodies from Article