Characterization of Atg38 and NRBF2, a fifth subunit of the autophagic Vps34/PIK3C3 complex.
Ohashi Y, Soler N, Garcia Ortegon M, Zhang L, Kirsten ML, Perisic O, Masson GR, Burke JE, Jakobi AJ, Apostolakis AA, Johnson CM, Ohashi M, Ktistakis NT, Sachse C, Williams RL
Autophagy. 2016 Nov;12(11):2129-2144. doi: 10.1080/15548627.2016.1226736. Epub 2016 Sep 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235700 | pOP795 | Protein expression His-taged HsNRBF2_FL in bacteria |
| 235701 | pOP796 | Protein expression of His-tagged HsNRBF2 MIT domain in bacteria |
| 235705 | pYO1025 | Expression of FL human VPS34 and human VPS15-ZZ in mammalian cells |
| 235734 | pYO395 | Expression of human VPS34 complex I in mammalian cells |
| 242481 | pYO780 | Expression of ScATG38 (FL)-2xEGFP under its own promoter |
| 242483 | pYO817 | Expression of ScAtg38 Δ(182 to 226)-2xEGFP under its own promoter |
| 242484 | pYO835 | Expression of ScAtg38 Δ(123 to 181)-2xEGFP under its own promoter |
| 242485 | pYO836 | Expression of ScAtg38 Δ(182 to 209)-2xEGFP under its own promoter |
| 242486 | pYO842 | Expression of ScAtg38 Δ(123 to 209)-2xEGFP under its own promoter |
| 242487 | pYO843 | Expression of ScAtg38 Δ(210 to 226)-2xEGFP under its own promoter |
| 242488 | pYO785 | Expression of ScAtg38 (FL)-3xFLAG under its own promoter |
| 242495 | pOP775 | Bacterial expression of GST-ScAtg38 (1-78) |
| 242496 | pOP768 | Bacterial expression of GST-ScAtg38 (87-226) |
| 242497 | pYO1089 | Bacterial expression of GST-ScAtg38 Δ(123 to 226) |
| 242498 | pYO1090 | Bacterial expression of GST-ScAtg38 Δ(182 to 226) |
| 242499 | pYO1091 | Bacterial expression of GST-ScAtg38 Δ(123 to 181) |
| 242500 | pYO1092 | Bacterial expression of GST-ScAtg38 Δ(182 to 209) |
| 242501 | pYO1093 | Bacterial expression of GST-ScAtg38 Δ(123 to 209) |
| 242502 | pYO1094 | Bacterial expression of GST-ScAtg38 Δ(210 to 226) |
| 242503 | pYO1022 | Expression of ZZ-3xTEV-HsATG14 and ZZ-3xTEV-HsBECN1 in mammalian cells |