Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes.
Tremel S, Ohashi Y, Morado DR, Bertram J, Perisic O, Brandt LTL, von Wrisberg MK, Chen ZA, Maslen SL, Kovtun O, Skehel M, Rappsilber J, Lang K, Munro S, Briggs JAG, Williams RL
Nat Commun. 2021 Mar 10;12(1):1564. doi: 10.1038/s41467-021-21695-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235727 | pYO1262 | Protein expression of His6-RAB1A Q70L 1-203 in bacteria |
| 235738 | pJB78 | Protein expression of GST-TEV-HsRAB1A Q70L C26S C126S in bacteria for maleimide conjugation |
| 235740 | STp6 | Protein expression of human RAB5A Q79,C19S,C63S,S84TAG, 1-212-His6 in bacteria for unnatural amino acid incorporation |