Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes.
Tremel S, Ohashi Y, Morado DR, Bertram J, Perisic O, Brandt LTL, von Wrisberg MK, Chen ZA, Maslen SL, Kovtun O, Skehel M, Rappsilber J, Lang K, Munro S, Briggs JAG, Williams RL
Nat Commun. 2021 Mar 10;12(1):1564. doi: 10.1038/s41467-021-21695-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235702 | pYO1005 | Expression of FL human UVRAG in mammalian cells |
| 235704 | pYO1015 | Expression of FL human VPS15-ZZ in mammalian cells |
| 235709 | pYO1274 | Expression of FL human VPS34 REIE > AAAA mutant in mammalian cells |
| 235710 | pYO1296 | Expression of mCherry-human RAB5A Q79L in mammalian cells |
| 235712 | pYO1280 | Expression of FL human VPS34-EGFP in mammalian cells |
| 235717 | pYO350 | Expression of FL human VPS15 in mammalian cells |
| 235725 | pYO1124 | Expression of human UVRAG (1-464) fused to human ATG14L BATS (413-492) in mammalian cells |
| 235727 | pYO1262 | Protein expression of His6-RAB1A Q70L 1-203 in bacteria |
| 235737 | pJB180 | Expression of mCherry- human RAB1A Q70L in mammalian cells |
| 235738 | pJB78 | Protein expression of GST-TEV-HsRAB1A Q70L C26S C126S in bacteria for maleimide conjugation |
| 235740 | STp6 | Protein expression of human RAB5A Q79,C19S,C63S,S84TAG, 1-212-His6 in bacteria for unnatural amino acid incorporation |
| 235741 | pYO1300 | Expression of VPS34 (REIE to AAAA)_FL - EGFP in mammalian cells |