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Roger Williams Lab: Williams Lab Plasmids 2025

Unpublished

Plasmids from Article

ID Plasmid Purpose
235703pYO1008Expression of FL human VPS34 in mammalian cells
235707pYO1100Expression of FL human ATG14L in mammalian cells
235711pYO1344Expression of FL human VPS34 R199E E200R E202R mutant in mammalian cells
235713pYO1356Expression of FL human VPS34 R199E E200R E202R muntant-EGFP in mammalian cells
235714pYO1846Expression of mCherry-human Beclin 1 in mammalian cells
235715pYO1847Expression of FL human VPS15 S579D_H580D_T583E mutant -ZZ in mammalian cells
235720pYO634Expression of FL yeast VPS15-EGFP under its own promoter in yeast
235721pYO1880Expression of FL yeast VPS15 S622D_H623D_T626E mutant -EGFP under its own promoter in yeast
235739pYO1510Protein expression of mCherry-14 x His in bacteria
235745pYO555Protein expression of KmKog1 in yeast
235767pYO1920Protein expression of ScVPS34 untagged + ScVPS15 S622D_H623E_T626E-ZZ in yeast
235769pYO1919Protein expression of 6xHis ScVPS21 (Q66L)-EGFP

Antibodies from Article