Structure and flexibility of the endosomal Vps34 complex reveals the basis of its function on membranes.
Rostislavleva K, Soler N, Ohashi Y, Zhang L, Pardon E, Burke JE, Masson GR, Johnson C, Steyaert J, Ktistakis NT, Williams RL
Science. 2015 Oct 9;350(6257):aac7365. doi: 10.1126/science.aac7365.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235729 | pYO225 | Protin expression of FL yeast VPS15 -ZZ in yeast |
| 235731 | pYO290 | Protein expression in FL yeast VPS34 D731N mutant in yeast |
| 235732 | pYO411 | Protein expression of ZZ-FL yeast Atg14 in yeast |
| 235733 | pYO379 | Protein expression of VPS34 + VPS15-ZZ + ZZ-VPS30 (all yeast) in yeast |
| 235742 | pYO1125 | Protein expression of GST-tagged PX domain (Cys at 2) in bacteria |
| 235747 | pYO69 | Protein expression of FL ScVPS34 in yeast |
| 235748 | pYO361 | Protein expression of ScVPS34 HELCAT |
| 235749 | pYO227 | Protein expression of ZZ-FL ScVPS30 |
| 235750 | pYO359 | Protein expression of FL ScVPS34 and FL ScVPS15-ZZ |
| 235753 | pYO734 | Protein expression of ScVPS30 and ZZ-ScVPS38 |
| 235754 | pYO831 | Protein expression of ZZ-ScVPS38 (1-212F) |
| 235755 | pYO832 | Protein expression of ScVPS30 (1-215E), untagged |
| 235757 | pYO478 | Expression of FL ScVPS34-3xFLAG under its own promoter |
| 235759 | pYO760 | Expression of ScVPS38 delta C2 (1-154)-x3FLAG under its own promoter |
| 235760 | pYO761 | Expression of ScVPS38 delta BARA2 (320-440)-x3FLAG under its own promoter |
| 235761 | pYO764 | Expression of ScVPS38-EGFP under its own promoter |
| 235762 | pYO765 | Expression of ScVPS38 delta C2 (1-154) -EGFP under its own promoter |
| 235763 | pYO766 | Expression of ScVPS38 delta BARA2 (320-440)-EGFP under its own promoter |
| 235764 | pYO932 | Expression of ScVPS34 HELCAT (268-875)-3xFLAG under its own promoter |
| 235765 | pYO933 | Expression of ScVPS34 delta C154-V202-3xFLAG under its own promoter |
| 235766 | pYO934 | Expression of ScVPS34 delta CBR1 L31-D55-3xFLAG undeer its own promoter |