Phosphorylation-State Modulated Binding of HSP70: Structural Insights and Compensatory Protein Engineering

Stewart M, Paththamperuma C, McCann C, Cottingim K, Zhang H, DelVecchio R, Peng I, Fennimore E, Nix JC, Saeed MN, George K, Makaroff M, Colie M, Paulakonis E, Almeida MF, Afolayan AJ, Brown NG, Page RC, Schisler JC
bioRxiv 2025.02.17.637997 Article

Plasmids from Article

ID	Plasmid	Purpose
236052	pAD000-CHIP-K30A-myc-His	Mammalian expression of human CHIP with the synthetic K30A substitution and C-terminal myc-His tag
236053	pAD120-CHIP-G132N-myc-His	Mammalian expression of human CHIP with the synthetic G132N substitution and C-terminal myc-His tag
236054	pAD100-CHIP-myc-His	Mammalian expression of human CHIP with a C-terminal myc-His tag
236055	pHE111-3xFlag-HSP70	Mammalian expression of human HSP70 with a N-terminal 3x-FLAG tag
236065	pSmBit-CHIP-G132N	SmBit-CHIP expression with G132N substitution
236066	pSmBit-CHIP-K30A	SmBit-CHIP expression with K30A substitution