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Phosphorylation-State Modulated Binding of HSP70: Structural Insights and Compensatory Protein Engineering

Stewart M, Paththamperuma C, McCann C, Cottingim K, Zhang H, DelVecchio R, Peng I, Fennimore E, Nix JC, Saeed MN, George K, Makaroff M, Colie M, Paulakonis E, Almeida MF, Afolayan AJ, Brown NG, Page RC, Schisler JC
bioRxiv 2025.02.17.637997 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
236052pAD000-CHIP-K30A-myc-HisMammalian expression of human CHIP with the synthetic K30A substitution and C-terminal myc-His tag
236053pAD120-CHIP-G132N-myc-HisMammalian expression of human CHIP with the synthetic G132N substitution and C-terminal myc-His tag
236054pAD100-CHIP-myc-HisMammalian expression of human CHIP with a C-terminal myc-His tag
236055pHE111-3xFlag-HSP70Mammalian expression of human HSP70 with a N-terminal 3x-FLAG tag

Antibodies from Article