Retraction of Astrocyte Leaflets From the Synapse Enhances Fear Memory.
Badia-Soteras A, Heistek TS, Kater MSJ, Mak A, Negrean A, van den Oever MC, Mansvelder HD, Khakh BS, Min R, Smit AB, Verheijen MHG
Biol Psychiatry. 2023 Aug 1;94(3):226-238. doi: 10.1016/j.biopsych.2022.10.013. Epub 2022 Oct 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240094 | pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-Seq1 | GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes |
| 240865 | pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-seq2 | GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes |
| 241026 | pAAV- GfaABC1D::Cas9-HA-sgRNA-Non-Targeting | GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of a non-targeting sgRNA; used as control for SaCas9 based knock-outs in murine astrocytes |