PAM-interacting domain turn-helix 51 motifs can improve Cas9-SpRY activity.
Eggenschwiler R, Hoffmann T, Dmytrenko O, Opitz M, Ackel-Zakour M, Wang P, McCallan SA, Fraguas-Eggenschwiler M, Niemann H, Patronov A, Beisel CL, Cantz T
Nucleic Acids Res. 2025 Aug 11;53(15):gkaf782. doi: 10.1093/nar/gkaf782.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235989 | iSM_PPL2-IP | Expresses iSM_PPL2 in mammalian cells |
| 235990 | iSM-IP | Expresses iSM (iSpyMac) in mammalian cells |
| 235991 | SpRY_ATH51-IP | Expresses SpRY_ATH51 in mammalian cells |
| 235992 | SpRY_MTH51-IP | Expresses SpRY_MTH51 in mammalian cells |
| 235993 | SpRY_SlTH51-IP | Expresses SpRY_SlTH51 in mammalian cells |
| 235994 | SpRY-IP | Expresses Cas9-SpRY in mammalian cells |
| 235995 | PB-PE iSM | Expresses iSM (iSpyMac) prime editor and pegRNA in mammalian cells |
| 235996 | PB-PE iSM_PPL2 | Expresses iSM_PPL2 prime editor and pegRNA in mammalian cells |
| 235998 | PB-PE SpRY_ATH51 | Expresses SpRY_ATH51 prime editor and pegRNA in mammalian cells |