Lysosomal PIP(3) revealed by genetically encoded lipid biosensors.
Sahan AZ, Chen M, Su Q, Li Q, Wang D, Zhang J
Proc Natl Acad Sci U S A. 2025 Apr;122(13):e2426929122. doi: 10.1073/pnas.2426929122. Epub 2025 Mar 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235999 | pcDNA3-InPTapp-Kras | Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to plasma membrane. |
| 236000 | pcDNA3-Lyso-InPTapp | Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to lysosome surface. |
| 236001 | pcDNA3-InPGrp-Kras | Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to plasma membrane. |
| 236002 | pcDNA3-Lyso-InPGrp | Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to lysosome surface. |
| 236003 | pcDNA3-Endo-InPGrp | Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to endosomes. |
| 236004 | pcDNA3-Lyso-INPP5E | Lysosome-targeted inositol polyphosphate 5 phosphatase (INPP5E); hydrolyzes 5-phosphate in PI(3,4,5)P3 and PI(4,5)P2. |
| 236005 | pcDNA3-Endo-INPP5E | Endosome-targeted inositol polyphosphate 5 phosphatase (INPP5E); hydrolyzes 5-phosphate in PI(3,4,5)P3 and PI(4,5)P2. |