The concentration of single-stranded DNA-binding proteins is a critical factor in recombinase polymerase amplification (RPA), as revealed by insights from an open-source system.
Cordoba-Andrade F, Peralta-Castro A, Garcia-Medel PL, Castro-Torres E, Gonzalez-Gonzalez R, Castro-Lara AY, Mora Garduno JD, Raygoza CD, Baruch-Torres N, Penafiel-Ayala A, Diaz-Quezada C, Cardona-Felix CS, Guzman Chavez F, Trasvina-Arenas CH, Sotelo-Mundo RR, Xoconostle-Cazares B, Martinez-Antonio A, Brieba de Castro LG
PeerJ. 2025 Aug 13;13:e19758. doi: 10.7717/peerj.19758. eCollection 2025.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 236044 | pET19b-pps-gp32 | gp32, synthetic gene |
| 236045 | pET19b-pps-UvsY | pET19b-pps-UvsY |
| 236046 | pET19b-pps-UvsX | pET19b-pps-UvsX |
| 236048 | pET19pps-EndoIV(Thermus Thermophilus) | pET19pps-APE1(Thermus Thermophilus) |