UltraCAST: A Flexible All-In-One Suicide Vector for Modifying Bacterial Genomes Using a CRISPR-Associated Transposon.

VanDieren AJ, Barrick JE
MicroPubl Biol. 2025 Aug 2;2025:10.17912/micropub.biology.001721. doi: 10.17912/micropub.biology.001721. eCollection 2025. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
236111	ultra CAST	Encodes sfGFP BbsI dropout for cloning in guide RNA sequence, encodes mScarlet BsmBI/BsaI dropout for cloning in cargo sequence
236186	pBTK1062	BsaI compatible kanamycin resistance cassette
236187	pBTK1126	BsaI compatible gentamicin resistance cassette