A toolbox for ablating excitatory and inhibitory synapses.
Bareghamyan A, Deng C, Daoudi S, Yadav SC, Lu X, Zhang W, Campbell RE, Kramer RH, Chenoweth DM, Arnold DB
eLife. 2025 Apr 29;13:RP103757. doi: 10.7554/eLife.103757.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 236112 | tagRFP-GPHN.FingR-HaloTag | Half of chemically inducible GFE3. When co-expressed with 236113 can degrade inhibitory synapses in presence of TH. (trimethoprim-HaloTag ligand). |
| 236113 | eDHFR-E3 | Half of chemically inducible GFE3. When co-expressed with 236112 can degrade inhibitory synapses in presence of TH. (trimethoprim-HaloTag ligand). |
| 236114 | TRE-PFE3-HA | Tet inducible PFE3 for ablating excitatory synapses. |
| 236178 | AAV-CAG-PFE3-P2A-GFP | AAV version of constitutive PFE3 for ablating excitatory synapses. |
| 236180 | AAV-TRE-PFE3-P2A-GFP | AAV version of Tet inducible PFE3 for ablating excitatory synapses. |
| 236181 | AAV-CAG-RandE3-P2A-GFP | AAV version of constitutive control plasmid |
| 236182 | PBP-E3-HA | Other half of photoactivatable GFE3. When co-expressed with tagRFP-GPHN.FingR-PhoCl (Plasmid #236183), ablates inhibitory synapses after exposure to 400 nm light. |
| 236183 | tagRFP-GPHN.FingR-PhoCl | Half of photoactivatable GFE3. When co-expressed with PBP-E3-HA (Plasmid #236182), ablates inhibitory synapses after exposure to 400 nm light. |