Model-Based Optimization of a qCRISPRi Circuit for Dynamic Control of Metabolic Pathways.
Golla SA, Abo-Hashesh M, Gupta D, Liu Y, Mahadevan R
ACS Synth Biol. 2025 Oct 17;14(10):3890-3898. doi: 10.1021/acssynbio.5c00095. Epub 2025 Sep 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 236036 | pQdCas12a.empty | The plasmid pQdCas12a.empty expresses the dCas12a endonuclease. |
| 236037 | pQdCas12a.luxR(mut)-empty | The plasmid pQdCas12a.luxR(mut)-empty expresses the dCas12a endonuclease. Additionally, it contains a mutation in the luxR gene. |
| 236038 | pQdCas12a.sggfp(A) | The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. |
| 236039 | pQdCas12a.luxR(mut)-sggfp(A) | The plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene. |
| 236040 | pQdCas12a.sggfp(B) | The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. |
| 236041 | pQdCas12a.luxR(mut)-sggfp(B) | The plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene. |
| 236042 | pQdCas12a.sggfp(C) | The plasmid pQdCas12a.sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. |
| 236043 | pQdCas12a.luxR(mut)-sggfp(C) | The plasmid pQdCas12a.luxR(mut)-sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene. |
| 236184 | pQdCas9-sggfp | The plasmid pQdCas9-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the gfp gene. |
| 236185 | pQdCas9.luxR(mut)-sggfp | The plasmid pQdCas9.luxR(mut)-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the GFP gene. Additionally, this plasmid contains a mutation in the luxR gene. |