Expanding the Yeast MoClo Toolkit: gene expression control parts for Saccharomyces cerevisiae tested in industrially relevant conditions.
Blick E, Nygard Y
Synth Biol (Oxf). 2025 Dec 4;10(1):ysaf018. doi: 10.1093/synbio/ysaf018. eCollection 2025.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 236215 | pEB0_11_5xQUAS_CYC1cp | Plasmid encoding 5xQUAS_CYC1cp (five repeats of the binding sequence of QF directly upstream of the CYC1 core promoter) as a Type 2 part to be used in the Dueber Yeast MoClo Toolkit. |
| 236216 | pEB0_13_PGK1p-QF-ACT1t | Plasmid encoding PGK1p-QF2-ACT1t as a combined Type 234 part to be used in the Dueber Yeast MoClo Toolkit. |
| 236217 | pEB0_14_QS | Plasmid encoding QS as a Type 3 part to be used in the Dueber Yeast MoClo Toolkit. |
| 236220 | pEB2_14_Q-system-QF2-ctrl | Control for Q-system in pEB2_15: Plasmid with QF2 under PGK1p and mTurquoise2 under 5xQUAS_CYC1cp with homologous regions for genome integration into HO locus. |
| 236221 | pEB2_15_Q-system | Q-system: Plasmid with QS under HHF2p, QF2 under PGK1p and mTurquoise2 under 5xQUAS_CYC1cp with homologous regions for genome integration into HO locus. |
| 236222 | pEB2_17_Q-system-ctrl | Control for Q-system in pEB2_15: Plasmid with mTurquoise2 under 5xQUAS_CYC1cp with homologous regions for genome integration into HO locus. |