Expanding the Yeast MoClo Toolkit: gene expression control parts for Saccharomyces cerevisiae tested in industrially relevant conditions.

Blick E, Nygard Y
Synth Biol (Oxf). 2025 Dec 4;10(1):ysaf018. doi: 10.1093/synbio/ysaf018. eCollection 2025. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
236220	pEB2_14_Q-system-QF2-ctrl	Control for Q-system in pEB2_15: Plasmid with QF2 under PGK1p and mTurquoise2 under 5xQUAS_CYC1cp with homologous regions for genome integration into HO locus.
236221	pEB2_15_Q-system	Q-system: Plasmid with QS under HHF2p, QF2 under PGK1p and mTurquoise2 under 5xQUAS_CYC1cp with homologous regions for genome integration into HO locus.
236222	pEB2_17_Q-system-ctrl	Control for Q-system in pEB2_15: Plasmid with mTurquoise2 under 5xQUAS_CYC1cp with homologous regions for genome integration into HO locus.