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Vsevolod Gurevich Lab: Expression of Untagged Arrestins in E. coli and Their Purification.

Vishnivetskiy SA, Zhan X, Gurevich VV
Curr Protoc. 2023 Sep;3(9):e832. doi: 10.1002/cpz1.832. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
236262BtARR1Bacterial expression of bovine arrestin-1 with inserted GGT codon after starting ATG
236263BtARR1 R175EBacterial expression of bovine arrestin-1 R175E with inserted GGT codon after starting ATG
236264BtARR1 (1-378)Bacterial expression of bovine arrestin-1 (1-378) E379Stop
236266BtARR3 longBacterial expression of bovine arrestin-3 long isoform
236267BtARR2 shortBacterial expression of bovine arrestin-2 short isoform
236268BtARR2 longBacterial expression of bovine arrestin-2 long isoform
236269BtARR2 long (1-382)Bacterial expression of bovine arrestin-2 long isoform (1-382) D383Stop
236270BtARR2 long (1-393)Bacterial expression of bovine arrestin-2 long isoform (1-393) Q394Stop
236272BtARR3 short (1-392)Bacterial expression of bovine arrestin-3 short (1-392) L393Stop

Antibodies from Article