CRISPR-array-mediated imaging of non-repetitive and multiplex genomic loci in living cells.
Yang LZ, Min YH, Liu YX, Gao BQ, Liu XQ, Huang Y, Wang H, Yang L, Liu ZJ, Chen LL
Nat Methods. 2024 Sep;21(9):1646-1657. doi: 10.1038/s41592-024-02333-3. Epub 2024 Jul 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 236361 | pHAGE-IRES-puro-NLS-dAsCas12a-EGFP-NLS-3xFlag | Overexpression of dAsCas12a in human cells |
| 236362 | pHAGE-IRES-puro-NLS-HyperdAsCas12a-EGFP-NLS-3xFlag | Overexpression of HyperdAsCas12a in human cells |
| 236363 | pHAGE-IRES-puro-NLS-endAsCas12a-EGFP-NLS-3xFlag | Overexpression of endAsCas12a in human cells |
| 236368 | pHAGE-IRES-puro-NLS-HyperdLbCas12a-StayGold-NLS-3xFlag | Overexpression of HyperdLbCas12a in human cells |
| 236369 | pCAG-48xCCAT1 array | Expression of array of 48 crRNAs targeting CCAT1 |
| 236370 | pHAGE-EFS-Halo-NLS-tDeg | Overexpression of Halo-NLS-tDeg in human cells |
| 236371 | pHAGE-EFS-MCP-3XBFP-2xNLS | Overexpression of MCP-3XBFP-2xNLS in human cells |
| 236372 | pHAGE-EFS-MCP-3XBFP-NLS | Overexpression of MCP-3XBFPnls in human cells |
| 236373 | pGL3-U6-aptamer | crRNA array expression |
| 236375 | pHAGE-IRES-puro-NLS-dFnCas12a-EGFP-NLS-3xFlag | Overexpression of dFnCas12a in human cells |
| 236376 | pHAGE-IRES-puro-NLS-HyperdFnCas12a-EGFP-NLS-3xFlag | Overexpression of HyperdFnCas12a in human cells |
| 236377 | pCAG-48xHSPA1A array | Expression of array of 48 crRNAs targeting HSPA1A |
| 236378 | pCAG-48xHSPA1B array | Expression of array of 48 crRNAs targeting HSPA1B |
| 236379 | pCAG-48xNFIL3 array | Expression of array of 48 crRNAs targeting NFIL3 |
| 236380 | pCAG-36XMYC array | Expression of array of 36 crRNAs targeting MYC |
| 236381 | pCAG-48xS100A10 array | Expression of array of 48 crRNAs targeting S100A10 |
| 236382 | pCAG-48xEFNA1 array | Expression of array of 48 crRNAs targeting EFNA1 |
| 236383 | pCAG-48xGPBP1 array | Expression of array of 48 crRNAs targeting GPBP1 |
| 236384 | pCAG-48xH3C1 array | Expression of array of 48 crRNAs targeting H3C1 |
| 236386 | pHAGE-IRES-puro-NLS-HyperdLbCas12a-NLS-3xFlag | Overexpression of HyperdLbCas12a in human cells |
| 236388 | pHAGE-EFS-PCP-3xSNAP-NLS | Overexpression of PCP-3xSNAP in human cells |