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Cingulin and paracingulin tether myosins-2 to junctions to mechanoregulate the plasma membrane.

Rouaud F, Huang W, Flinois A, Jain K, Vasileva E, Di Mattia T, Mauperin M, Parry DAD, Dugina V, Chaponnier C, Mean I, Montessuit S, Mutero-Maeda A, Yan J, Citi S
J Cell Biol. 2023 Jul 3;222(7):e202208065. doi: 10.1083/jcb.202208065. Epub 2023 May 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
236506GFP-cCGN-MycFor expression of CGN (dog) in mammalian cells
236512Strep-His-Tev-hCGNFor expression of Cingulin (human) in insect cells
236516GFP-cCGNL1-MycFor expression of Paracingulin (dog) in mammalian cells
236517GFP-cCGNL1-HAFor expression of Paracingulin (dog) in mammalian cells
236519GFP-mCGNL1For expression of Paracingulin (mouse) in mammalian cells
236520Strep-His-Tev-hCGNL1For expression of Paracingulin (human) in insect cells
236531HA-mNM2AFor expression of NM2A (mouse) in mammalian cells
236532HA-cNM2A-FlagFor expression of NM2A (dog) - with HA and FLAG tags in mammalian cells
236533HA-hNM2BFor expression of NM2B (human) in mammalian cells
236534HA-cNM2B-FlagFor expression of NM2B (dog) - with HA and FLAG tags in mammalian cells
236535HA-cNM2CFor expression of NM2C (dog) in mammalian cells

Antibodies from Article