Cingulin and paracingulin tether myosins-2 to junctions to mechanoregulate the plasma membrane.
Rouaud F, Huang W, Flinois A, Jain K, Vasileva E, Di Mattia T, Mauperin M, Parry DAD, Dugina V, Chaponnier C, Mean I, Montessuit S, Mutero-Maeda A, Yan J, Citi S
J Cell Biol. 2023 Jul 3;222(7):e202208065. doi: 10.1083/jcb.202208065. Epub 2023 May 19.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
236506 | GFP-cCGN-Myc | For expression of CGN (dog) in mammalian cells |
236512 | Strep-His-Tev-hCGN | For expression of Cingulin (human) in insect cells |
236516 | GFP-cCGNL1-Myc | For expression of Paracingulin (dog) in mammalian cells |
236517 | GFP-cCGNL1-HA | For expression of Paracingulin (dog) in mammalian cells |
236519 | GFP-mCGNL1 | For expression of Paracingulin (mouse) in mammalian cells |
236520 | Strep-His-Tev-hCGNL1 | For expression of Paracingulin (human) in insect cells |
236531 | HA-mNM2A | For expression of NM2A (mouse) in mammalian cells |
236532 | HA-cNM2A-Flag | For expression of NM2A (dog) - with HA and FLAG tags in mammalian cells |
236533 | HA-hNM2B | For expression of NM2B (human) in mammalian cells |
236534 | HA-cNM2B-Flag | For expression of NM2B (dog) - with HA and FLAG tags in mammalian cells |
236535 | HA-cNM2C | For expression of NM2C (dog) in mammalian cells |