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Central regulation of feeding and body weight by ciliary GPR75.

Jiang Y, Xun Y, Zhang Z
J Clin Invest. 2024 Aug 13;134(19):e182121. doi: 10.1172/JCI182121. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
236744pBOB-3xFlag-mGpr75Express 3xFlag N-terminal tagged wild-type mouse GPR75 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
236745pBOB-3xFlag-mGpr75-L144PExpress 3xFlag N-terminal tagged mouse GPR75-L144P mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
236749pCMV-BirA-HA-Tulp3Express BirA and HA tagged TULP3 transiently via transfection in mammalian cells.
236750pBOB-HA-GnaqExpress HA N-terminal tagged mouse Gq transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
236751pGL3-MRE/CRE/SREUse as a MRE/CRE/SRE reporter for luciferase assays to measure GPCR activation.

Antibodies from Article