Central regulation of feeding and body weight by ciliary GPR75.
Jiang Y, Xun Y, Zhang Z
J Clin Invest. 2024 Aug 13;134(19):e182121. doi: 10.1172/JCI182121.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 236744 | pBOB-3xFlag-mGpr75 | Express 3xFlag N-terminal tagged wild-type mouse GPR75 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells. |
| 236745 | pBOB-3xFlag-mGpr75-L144P | Express 3xFlag N-terminal tagged mouse GPR75-L144P mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells. |
| 236746 | pBOB-3xFlag-hGpr75 | Express 3xFlag N-terminal tagged wild-type human GPR75 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells. |
| 236747 | pBOB-3xFlag-hGpr75-p.Ala110fs | Express 3xFlag N-terminal tagged human GPR75 p.Ala110fs mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells. |
| 236748 | pBOB-3xFlag-hGpr75-p.Gln234* | Express 3xFlag N-terminal tagged human GPR75 p.Gln234* mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells. |
| 236749 | pCMV-BirA-HA-Tulp3 | Express BirA and HA tagged TULP3 transiently via transfection in mammalian cells. |
| 236750 | pBOB-HA-Gnaq | Express HA N-terminal tagged mouse Gq transiently via transfection or stably via lentivirus-mediated infection in mammalian cells. |
| 236751 | pGL3-MRE/CRE/SRE | Use as a MRE/CRE/SRE reporter for luciferase assays to measure GPCR activation. |