Multiplexed genetic tags for electron and fluorescence microscopy.

Berezin O, Piovesan A, Graf R, Samara E, Sigmund F, Westmeyer GG
Nat Protoc. 2025 Nov 19. doi: 10.1038/s41596-025-01260-7. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
236775	HA_DD-N-mScarlet-I-QtSig	Expresses the red fluorescent protein mScarlet-I fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.
236776	HA_DD-N-mTagBFP2-QtSig	Expresses the blue fluorescent protein mTagBFP2 fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.
236777	DD-N-nLuc-QtSig	Expresses the luminescent protein nLuc fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.
238894	1M-Qt-eUnaG-TGA-rt20s-anti-mCherry(LaM-4)	EMcapsulin class with a eUnaG fusion and an anti-mCherry intrabody (LaM-4) fusion preceded by a TGA stop codon and the rt20s readthrough motif.