Improved base editing and functional screening in Leishmania via co-expression of the AsCas12a ultra variant, a T7 RNA polymerase, and a cytosine base editor.

Herrmann May N, Cao A, Schmid A, Link F, Arias-Del-Angel J, Meiser E, Beneke T
eLife. 2025 Feb 24;13:RP97437. doi: 10.7554/eLife.97437. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
236778	pTB105	Backbone for cloning sgRNAs to be used with CRISPR Cas9 cytosine base editor. Expressed from the 18S rRNA SSU locus in Leishmania. Contains puromycin selection marker.
236779	pTB106-Hyg	Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and hygromycin selection marker. The CBE contains a ssDNA-DBD from the L. major RAD51 protein. This plasmid is transfected as episome.
236782	pTB107-Phleo	Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and phleomycin selection marker. The CBE contains a ssDNA-DBD from the H. sapiens RAD51 protein. This plasmid is transfected as episome.