Improved base editing and functional screening in Leishmania via co-expression of the AsCas12a ultra variant, a T7 RNA polymerase, and a cytosine base editor.
Herrmann May N, Cao A, Schmid A, Link F, Arias-Del-Angel J, Meiser E, Beneke T
eLife. 2025 Feb 24;13:RP97437. doi: 10.7554/eLife.97437.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 236778 | pTB105 | Backbone for cloning sgRNAs to be used with CRISPR Cas9 cytosine base editor. Expressed from the 18S rRNA SSU locus in Leishmania. Contains puromycin selection marker. |