Self-assembling protein nanoparticles for cytosolic delivery of nucleic acids and proteins.

Eweje F, Ibrahim V, Shajii A, Walsh ML, Ahmad K, Alrefai A, Miyasato D, Davis JR, Ham H, Li K, Roehrl M, Haller CA, Liu DR, Chen J, Chaikof EL
Nat Biotechnol. 2025 May 15. doi: 10.1038/s41587-025-02664-2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
237223	pQE-80L-V4-ELP-EEP13	Expresses micelle-forming elastin-like polypeptide (ELP)-endosomal escape peptide (EEP) fusion construct in bacteria
237224	pET-Cre-LPETG-6xHis	Expresses LPETG-tagged Cre recombinase in bacteria
237225	pET-hIRF5-V5(4D)-LPETG	Expresses LPETG-tagged, constitutively active human IRF5 variant in bacteria