Functional and structural profiling of circulation via genetically encoded modular fluorescent probes
Vittani M, Lee AB, Wang X, Hiraoka Y, Konno A, Knak PG, Kusk P, Nagao M, Asiminas A, Courtin J, Putranto MF, Nasu Y, Tsuno S, Ueda K, Osuga Y, Tsuboi T, Bienvenu T, Terunuma M, Hirai H, Nedergaard M, Tanaka K, Hirase H
bioRxiv 2025.03.18.643859
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234541 | pAAV-P3-IgKL-SpyCatcher003-SEP | to express a pH sensor tagged with SpyCatcher in the liver |
| 234543 | pAAV-P3-IgKL-SpyCatcher003-mNeonGreen | to express a green fluorescent protein tagged with SpyCatcher in the liver |
| 234544 | pAAV-P3-IgKL-SpyCatcher003-GINKO2 | to express a potassium sensor tagged with SpyCatcher in the liver |
| 234545 | pAAV-mGFAP(ABC1D)-ECS-SpyCatcher003 | to express SpyCatcher on the extracellular side of astrocytic plasmamembrane |
| 234546 | pAAV-mGFAP(ABC1D)-ECS-dSpyCatcher003 | to express dSpyCatcher on the extracellular side of astrocytic plasmamembrane |
| 234963 | pAAV-CAG-DIO-ECS-dSpyCatcher003 | Cre-dependent ECS-facing destabilized SpyCatcher003 (facing the extracellular space) |