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Antibody-lectin chimeras for glyco-immune checkpoint blockade.

Stark JC, Gray MA, Ibarlucea-Benitez I, Lustig M, Bond A, Cho B, Govil I, Luu T, Priestley MJ, Veth TS, Errington WJ, Bruncsics B, Ribi MK, Williams LA, Sarkar CA, Wisnovsky S, Riley NM, Morrissey MA, Valerius T, Ravetch JV, Bertozzi CR.
Nat Biotechnol 2025 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
237364pTCB-trasHCknob6xHispTwist CMV BetaGlobin encoding trastuzumab heavy chain with knob Fc and 6xHis tag
237365pTCB-trasLCpTwist CMV BetaGlobin encoding trastuzumab light chain
237366TCB-Sig7FcLALAPGholeHApTwist CMV BetaGlobin encoding Siglec-7-Fc with LALA P329G hole Fc and HA tag
237367pTCB-Sig9FcholeHApTwist CMV BetaGlobin encoding Siglec-9-Fc with hole Fc and HA tag
237368pTCB-Sig7R124AFcholeHApTwist CMV BetaGlobin encoding Siglec-7-Fc R124A mutant with hole Fc and HA tag
237369pTCB-Sig9R120AFcholeHApTwist CMV BetaGlobin encoding Siglec-9-Fc R120A mutant with hole Fc and HA tag
237370pTCB-Sig10FcholeHApTwist CMV BetaGlobin encoding Siglec-10-Fc with hole Fc and HA tag
237371pTCB-rituxHCknob6xHispTwist CMV BetaGlobin encoding rituximab heavy chain with knob Fc and 6xHis tag
237372pTCB-rituxLCpTwist CMV BetaGlobin encoding rituximab light chain
237373pTCB-cetuxHCknob6xHispTwist CMV BetaGlobin encoding cetuximab heavy chain with knob Fc and 6xHis tag
237374pTCB-cetuxLCpTwist CMV BetaGlobin encoding cetuximab light chain
237375pTCB-magroHCLALAPGknob6xHispTwist CMV BetaGlobin encoding magrolimab heavy chain with LALA P329G knob Fc and 6xHis tag
237376pTCB-magroLCpTwist CMV BetaGlobin encoding magrolimab light chain
237377pTCB-nivoHCLALAPGknobHApTwist CMV BetaGlobin encoding nivolumab heavy chain with LALA P329G knob Fc and HA tag
237378pTCB-nivoLCpTwist CMV BetaGlobin encoding nivolumab light chain
237379pTCB-pembroHCLALAPGknobHApTwist CMV BetaGlobin encoding pembrolizumab heavy chain with LALA P329G knob Fc and HA tag
237380pTCB-pembroLCpTwist CMV BetaGlobin encoding pembrolizumab light chain
237381pTCB-Gal9NFcLALAPGhole6xHispTwist CMV BetaGlobin encoding Galectin-9 N terminal carbohydrate binding domain-Fc fusion with LALA P329G hole Fc and 6xHis tag
237382pTCB-Sig7FcholeHApTwist CMV BetaGlobin encoding Siglec-7-Fc with hole Fc and HA tag
237383pTCB-avelHCknob6xHispTwist CMV BetaGlobin encoding avelumab heavy chain with knob Fc and 6xHis tag
237384pTCB-avelLCpTwist CMV BetaGlobin encoding avelumab light chain

Antibodies from Article