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Structural basis for the dynamic regulation of mTORC1 by amino acids

Valenstein ML, Wranik M, Lalgudi PV, Linde-Garelli KY, Choi Y, Chivukula RR, Sabatini DM, Rogala KB
Nature (2025) (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
237416FLAG-sc-WDR59-SEC13Transient expression of N-terminally FLAG-tagged human WDR59-SEC13 "single chain" in the GATOR2 complex
237417sc-MIOS-SEH1LTransient expression of human MIOS-SEH1L "single chain" in the GATOR2 complex
237418sc-WDR24-SEH1LTransient expression of human WDR24-SEH1L "single chain" in the GATOR2 complex
237419CASTOR1-HA (D304A)Transient expression of C-terminally HA-tagged human CASTOR1 (D304A mutant)
237420HA-Sestrin2 (E451Q)Transient expression of N-terminally HA-tagged human Sestrin2 (E451Q)

Antibodies from Article