Discovery and engineering of retrons for precise genome editing.
Buffington JD, Kuo HC, Hu K, Chang YC, Javanmardi K, Voigt B, Li YR, Little ME, Devanathan SK, Xhemalce B, Gray RS, Finkelstein IJ
Nat Biotechnol. 2025 Oct 23. doi: 10.1038/s41587-025-02879-3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 237445 | pIF1079 | Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely. |