Wheat tandem kinase RWT4 directly binds a fungal effector to activate defense.
Sung YC, Li Y, Bernasconi Z, Baik S, Asuke S, Keller B, Fahima T, Coaker G
Nat Genet. 2025 Apr 14. doi: 10.1038/s41588-025-02162-w.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
237509 | pUC19::ZmUBI-RWT4Ca | Protoplast expression |
237510 | pUC19::ZmUBI-RWT4Cs | Protoplast expression |
237512 | pUC19::ZmUBI-RWT4Ca_Reg1 | Protoplast expression |
237513 | pUC19::ZmUBI-RWT4Ca_Reg2 | Protoplast expression |
237514 | pUC19::ZmUBI-RWT4Ca+C-Tail | Protoplast expression |
237515 | pUC19::ZmUBI-RWT4Ca_noKDup | Protoplast expression |
237516 | pUC19::ZmUBI-AvrPWT4 | Protoplast expression |
237517 | pUC19::ZmUBI-VirPWT4 | Protoplast expression |
237518 | pET28a:His-MBP-RWT4Ca | Recombinant protein expression |
237519 | pET28a:His-RWT4Ca | Recombinant protein expression |
237520 | pET28a:His-MBP-KD | Recombinant protein expression |
237521 | pET28a:His-MBP-PKD | Recombinant protein expression |
237522 | pET28a:GST-AvrPWT4 | Recombinant protein expression |
237523 | pET28a:GST-VirPWT4 | Recombinant protein expression |
237524 | pGADT7-RWT4Ca | Yeast two-hybrid |
237525 | pGADT7-RWT4Ca_Reg1 | Yeast two-hybrid |
237526 | pGADT7-RWT4Ca+C-Tail | Yeast two-hybrid |
237527 | pGBKT7-AvrPWT4 | Yeast two-hybrid |
237528 | pGBKT7-VirPWT4 | Yeast two-hybrid |
237531 | pTA7001-RWT4Ca_noKDup | Expression in Nicotiana benthamiana |
237532 | pTA7001-AvrPWT4-GFP | Expression in Nicotiana benthamiana |
237533 | pTA7001-VirPWT4-GFP | Expression in Nicotiana benthamiana |
237534 | pTA7001-RWT4Ca_KDup | Expression in Nicotiana benthamiana |
237535 | pET28a:His-MBP-RWT4Ca_noKDup | Recombinant protein expression |