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Wheat tandem kinase RWT4 directly binds a fungal effector to activate defense.

Sung YC, Li Y, Bernasconi Z, Baik S, Asuke S, Keller B, Fahima T, Coaker G
Nat Genet. 2025 Apr 14. doi: 10.1038/s41588-025-02162-w. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
237509pUC19::ZmUBI-RWT4CaProtoplast expression
237510pUC19::ZmUBI-RWT4CsProtoplast expression
237511pUC19::ZmUBI-RWT4Ca_K217R/K327RProtoplast expression
237512pUC19::ZmUBI-RWT4Ca_Reg1Protoplast expression
237513pUC19::ZmUBI-RWT4Ca_Reg2Protoplast expression
237514pUC19::ZmUBI-RWT4Ca+C-TailProtoplast expression
237515pUC19::ZmUBI-RWT4Ca_noKDupProtoplast expression
237516pUC19::ZmUBI-AvrPWT4Protoplast expression
237517pUC19::ZmUBI-VirPWT4Protoplast expression
237518pET28a:His-MBP-RWT4CaRecombinant protein expression
237519pET28a:His-RWT4CaRecombinant protein expression
237520pET28a:His-MBP-KDRecombinant protein expression
237521pET28a:His-MBP-PKDRecombinant protein expression
237522pET28a:GST-AvrPWT4Recombinant protein expression
237523pET28a:GST-VirPWT4Recombinant protein expression
237524pGADT7-RWT4CaYeast two-hybrid
237525pGADT7-RWT4Ca_Reg1Yeast two-hybrid
237526pGADT7-RWT4Ca+C-TailYeast two-hybrid
237527pGBKT7-AvrPWT4Yeast two-hybrid
237528pGBKT7-VirPWT4Yeast two-hybrid
237529pTA7001-RWT4CaExpression in Nicotiana benthamiana
237530pTA7001-RWT4CsExpression in Nicotiana benthamiana
237531pTA7001-RWT4Ca_noKDupExpression in Nicotiana benthamiana
237532pTA7001-AvrPWT4-GFPExpression in Nicotiana benthamiana
237533pTA7001-VirPWT4-GFPExpression in Nicotiana benthamiana
237534pTA7001-RWT4Ca_KDupExpression in Nicotiana benthamiana
237535pET28a:His-MBP-RWT4Ca_noKDupRecombinant protein expression

Antibodies from Article