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Ultrabright chemical labeling enables rapid neural connectivity profiling in large tissue samples.

Zhong S, Zhang X, Gao X, Li Z, Huang L, Guo Q, Gong R, Ren J, Luo M, Lin R
Neuron. 2025 Sep 18:S0896-6273(25)00657-9. doi: 10.1016/j.neuron.2025.08.022. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
237885pAAV-CAG-DIO-TurboIDAAV vector for Cre-dependent expression of TurboID
237886pAAV-CAG-DIO-seTurboIDAAV vector for Cre-dependent expression of seTurboID
237887pAAV-CAG-DIO-mem-seTurboIDAAV vector for Cre-dependent expression of membrane-tethered seTurboID
237888pAAV-hSyn-mem-seTurboIDAAV vector encoding membrane-tethered seTurboID under the control of human synapsin promoter
237889pAAV-TRE-fDIO-mem-seTurboID-IRES-tTAAmplifier AAV vector of Dual-AAV sparse labeling system encoding membrane-tethered seTurboID
237890pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-1Cre-KO AAV vector#1 containing SaCas9 and its sgRNA targeting Cre
237891pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-2Cre-KO AAV vector#2 containing SaCas9 and its sgRNA targeting Cre
237892pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-3Cre-KO AAV vector#3 containing SaCas9 and its sgRNA targeting Cre
240206pAAV-TRE-fDIO-seTurboID-IRES-tTAAmplifier AAV vector of Dual-AAV sparse labeling system encoding seTurboID

Antibodies from Article