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TIGR-Tas: A family of modular RNA-guided DNA-targeting systems in prokaryotes and their viruses.

Faure G, Saito M, Wilkinson ME, Quinones-Olvera N, Xu P, Flam-Shepherd D, Kim S, Reddy N, Zhu S, Evgeniou L, Koonin EV, Macrae RK, Zhang F
Science. 2025 Feb 27:eadv9789. doi: 10.1126/science.adv9789. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
238204pMW357Expresses untagged TaTasR from a PBAD promoter, followed by the native TIGR array (8 units)
238205pMW364Expresses untagged TasR from a PBAD promoter, followed by 3xRNA1 TIGR array
238207pMIIS794Expresses native TaTasR TIGR locus, TasR has premature stop codon. CmR
238208pMW363Expresses TasR with an N-terminal Twin-StrepII tag, followed by 3xRNA1 TIGR array. AmpR
238209pMIIS644Expresses native FpTasA TIGR locus. CmR
238212pMIIS1011Expresses ParTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.

Antibodies from Article