TIGR-Tas: A family of modular RNA-guided DNA-targeting systems in prokaryotes and their viruses.
Faure G, Saito M, Wilkinson ME, Quinones-Olvera N, Xu P, Flam-Shepherd D, Kim S, Reddy N, Zhu S, Evgeniou L, Koonin EV, Macrae RK, Zhang F
Science. 2025 Feb 27:eadv9789. doi: 10.1126/science.adv9789.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 238204 | pMW357 | Expresses untagged TaTasR from a PBAD promoter, followed by the native TIGR array (8 units) |
| 238205 | pMW364 | Expresses untagged TasR from a PBAD promoter, followed by 3xRNA1 TIGR array |
| 238206 | pMIIS793 | Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR |
| 238207 | pMIIS794 | Expresses native TaTasR TIGR locus, TasR has premature stop codon. CmR |
| 238208 | pMW363 | Expresses TasR with an N-terminal Twin-StrepII tag, followed by 3xRNA1 TIGR array. AmpR |
| 238209 | pMIIS644 | Expresses native FpTasA TIGR locus. CmR |
| 238210 | pMIIS638 | Expresses native SpTasH TIGR locus. CmR |
| 238211 | pMIIS928 | Expresses TaTasR with an N-terminal HA-NLS tag under CMV promoter in human cells. |
| 238212 | pMIIS1011 | Expresses ParTasR with an N-terminal HA-NLS tag under CMV promoter in human cells. |
| 238213 | pMIIS948-CXCR4 | TasR tigRNA expression to target CXCR4 under U6 promoter in human cells. |