Skip to main content
Addgene

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states.

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM
bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
238164pRCA360 - pBA904 Puro-T2A-GFPLentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
238167pRCA376 - pBA904 Puro-T2A-GFP NTC guide (pRCA360 backbone)Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
238168pRCA377 - pBA904 Puro-T2A-GFP CD55 CRISPRa guide 1 (pRCA360 backbone)Lentiviral CRISPR guide vector expressing CD55 targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
238169pRCA378 - pBA904 Puro-T2A-GFP CD45 CRISPRa guide 3 (pRCA360 backbone)Lentiviral CRISPR guide vector expressing PTPRC targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
238170pRCA594 - pBA904 Puro-T2A-BFP Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
238171pRCA595 - pBA904 Puro-T2A-mCherryLentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
238172pRCA803 - pBA904 Puro-T2A-GFP KLF5 g1 CRISPRa guide (pRCA360 backbone) 666Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
238173pRCA804 - pBA904 Puro-T2A-GFP KLF5 g2 CRISPRa guide (pRCA360 backbone) 665Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
238174pRCA805 - pBA904 Puro-T2A-GFP KLF4 g1 CRISPRa guide (pRCA360 backbone) 668Lentiviral CRISPR guide vector expressing a KLF4 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
238177pRCA810 - pBA904 Puro-T2A-GFP HES7 g2 CRISPRa guide (pRCA360 backbone) 674Lentiviral CRISPR guide vector expressing a HES7 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
238178pRCA815 - pBA904 Puro-T2A-GFP ZNF296 g5 CRISPRa guide (pRCA360 backbone) 695Lentiviral CRISPR guide vector expressing a ZNF296 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
238179pRCA816 - pBA904 Puro-T2A-GFP ZNF296 g6 CRISPRa guide (pRCA360 backbone) 694Lentiviral CRISPR guide vector expressing a ZNF296 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
238180pRCA892 - TFORF Empty Vector Puro-T2A-GFP (pRCA886 backbone)Lentiviral empty vector
238181pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone)Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter
238182pRCA881 - KLF4 Puro-T2A-GFP (pRCA847 backbone)Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter
238183pRCA918 - TFORF Empty Vector Puro-P2A-BFP (pRCA892 backbone)Lentiviral empty vector
238184pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845 backbone)Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter
238185pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter
238187pRCA895 - pBA904 Puro-T2A-BFP KLF4 g1 CRISPRa guide guide (pRCA594 backbone) 668Lentiviral CRISPR guide vector expressing a KLF4 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
238189pRCA938 - pBA904 Puro-T2A-GFP PLAGL1_g1 CRISPRa guide guide (pRCA360 backbone)Lentiviral CRISPR guide vector expressing a PLAGL1 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Antibodies from Article