Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states.
Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM
Nat Genet. 2025 Aug 6. doi: 10.1038/s41588-025-02284-1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 238164 | pRCA360 - pBA904 Puro-T2A-GFP | Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture |
| 238165 | pRCA422 - pHR-UCOE-SFFV-VPH-dcas9-p2a-mCherry | Express VPH-dCas9-P2A-mCherry in mammalian cells. Lentiviral |
| 238166 | pTMN140 - UCOE-SFFV-VPH-XTEN-dCas9-IRES2-BFP-T2A-Blast | Express VPH-dCas9 and BFP-T2A-BlasticidinR in mammalian cells. Lentiviral |
| 238167 | pRCA376 - pBA904 Puro-T2A-GFP NTC guide (pRCA360 backbone) | Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture |
| 238168 | pRCA377 - pBA904 Puro-T2A-GFP CD55 CRISPRa guide 1 (pRCA360 backbone) | Lentiviral CRISPR guide vector expressing CD55 targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture |
| 238169 | pRCA378 - pBA904 Puro-T2A-GFP CD45 CRISPRa guide 3 (pRCA360 backbone) | Lentiviral CRISPR guide vector expressing PTPRC targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture |
| 238170 | pRCA594 - pBA904 Puro-T2A-BFP | Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture |
| 238171 | pRCA595 - pBA904 Puro-T2A-mCherry | Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture |
| 238172 | pRCA803 - pBA904 Puro-T2A-GFP KLF5 g1 CRISPRa guide (pRCA360 backbone) 666 | Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture |
| 238173 | pRCA804 - pBA904 Puro-T2A-GFP KLF5 g2 CRISPRa guide (pRCA360 backbone) 665 | Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture |
| 238174 | pRCA805 - pBA904 Puro-T2A-GFP KLF4 g1 CRISPRa guide (pRCA360 backbone) 668 | Lentiviral CRISPR guide vector expressing a KLF4 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture |
| 238177 | pRCA810 - pBA904 Puro-T2A-GFP HES7 g2 CRISPRa guide (pRCA360 backbone) 674 | Lentiviral CRISPR guide vector expressing a HES7 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture |
| 238178 | pRCA815 - pBA904 Puro-T2A-GFP ZNF296 g5 CRISPRa guide (pRCA360 backbone) 695 | Lentiviral CRISPR guide vector expressing a ZNF296 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture |
| 238179 | pRCA816 - pBA904 Puro-T2A-GFP ZNF296 g6 CRISPRa guide (pRCA360 backbone) 694 | Lentiviral CRISPR guide vector expressing a ZNF296 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture |
| 238180 | pRCA892 - TFORF Empty Vector Puro-T2A-GFP (pRCA886 backbone) | Lentiviral empty vector |
| 238181 | pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone) | Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter |
| 238182 | pRCA881 - KLF4 Puro-T2A-GFP (pRCA847 backbone) | Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter |
| 238183 | pRCA918 - TFORF Empty Vector Puro-P2A-BFP (pRCA892 backbone) | Lentiviral empty vector |
| 238184 | pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845 backbone) | Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter |
| 238185 | pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone) | Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter |
| 238186 | pRCA893 - pBA904 Puro-T2A-BFP KLF5 g1 CRISPRa guide guide (pRCA594 backbone) 666 | Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture |
| 238187 | pRCA895 - pBA904 Puro-T2A-BFP KLF4 g1 CRISPRa guide guide (pRCA594 backbone) 668 | Lentiviral CRISPR guide vector expressing a KLF4 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture |
| 238188 | pRCA860 - pBA904 Puro-T2A-GFP EGR3 g1 CRISPRa guide guide (pRCA360 backbone) | Lentiviral CRISPR guide vector expressing a EGR3 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture |
| 238189 | pRCA938 - pBA904 Puro-T2A-GFP PLAGL1_g1 CRISPRa guide guide (pRCA360 backbone) | Lentiviral CRISPR guide vector expressing a PLAGL1 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture |