A conserved mechanism for the retrieval of polyubiquitinated proteins from cilia.
Lange SM, Bennett JA, Eisert RJ, Brown A
Cell. 2025 Nov 13;188(23):6492-6508.e18. doi: 10.1016/j.cell.2025.07.043. Epub 2025 Aug 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 238214 | pET156P CFAP36-3C-muGFP-10His (Sus scrofa) | Bacterial expression vector of porcine CFAP36 with C-terminal tags |
| 238215 | pET156P ARL2-3C-muGFP-10His (Sus scrofa) | Bacterial expression vector of porcine ARL2 with C-terminal tags |
| 238216 | pET156P ARL3-3C-muGFP-10His (Sus scrofa) | Bacterial expression vector of porcine ARL3 with C-terminal tags |
| 238217 | pET156P ARL6-3C-muGFP-10His (Sus scrofa) | Bacterial expression vector of porcine ARL6 with C-terminal tags |
| 238218 | pET156P CFAP36 [M1-E186]-muGFP (Sus scrofa) | Bacterial expression vector of porcine CFAP36 [M1-E186] with C-terminal tags |
| 238219 | pET156P CFAP36 [W10E]-3C-muGFP-10His (Sus scrofa) | Bacterial expression vector of porcine CFAP36 W10E mutant with C-terminal tags |
| 238220 | pET156P CFAP36 [S92E]-3C-muGFP-10His (Sus scrofa) | Bacterial expression vector of porcine CFAP36 S92E mutant with C-terminal tags |
| 238221 | pET156P CFAP36 [A123E]-3C-muGFP-10His (Sus scrofa) | Bacterial expression vector of porcine CFAP36 A123E mutant with C-terminal tags |
| 238222 | pET156P CFAP36 [S163E]-3C-muGFP-10His (Sus scrofa) | Bacterial expression vector of porcine CFAP36 S163E mutant with C-terminal tags |
| 238223 | pET156P CFAP36 [S180E]-3C-muGFP-10His (Sus scrofa) | Bacterial expression vector of porcine CFAP36 S180E mutant with C-terminal tags |
| 238224 | pET156P ARL3-3xFlag-CFAP36-10His (Sus scrofa) | Bacterial expression vector of porcine ARL3-3xFlag-CFAP36 fusion with C-terminal His tag |
| 238225 | pET156P ARL3-3xFlag-CFAP36 [S163E]-10His (Sus scrofa) | Bacterial expression vector of porcine ARL3-3xFlag-CFAP36 [S163E] fusion with C-terminal His tag |
| 238226 | pBR25 CFAP36-3C-mStayGold-2Strep crBSDr (C. reinhardtii) | CRISPR donor plasmid for C-terminal tagging of CFAP36 and insertion of Blasticidin resistance (C. reinhardtii) |
| 238227 | pBR25 CFAP36 [L438E, K442E]-3C-mStayGold-2Strep crBSDr (C. reinhardtii) | CRISPR donor plasmid for C-terminal tagging and knock-in of CFAP36 [L438E, K442E], and insertion of Blasticidin resistance (C. reinhardtii) |
| 238228 | pBR25 CFAP36 [A493E, K495A]-3C-mStayGold-2Strep crBSDr (C. reinhardtii) | CRISPR donor plasmid for C-terminal tagging and knock-in of CFAP36 [A493E, K495A], and insertion of Blasticidin resistance (C. reinhardtii) |
| 238229 | pBR25 CFAP36 [L438E, K442E, A493E, K495A]-3C-mStayGold-2Strep crBSDr (C. reinhardtii) | CRISPR donor plasmid for C-terminal tagging and knock-in of CFAP36 [L438E, K442E, A493E, K495A], and insertion of Blasticidin resistance (C. reinhardtii) |
| 238230 | pBR25 IFT70 [A331R, A335R] crSpecR (C. reinhardtii) | CRISPR donor plasmid for C-terminal knock-in of IFT70 [A331R, A335R] and insertion of Spectinomycin resistance (C. reinhardtii) |