One-Step Purification and N-Terminal Functionalization of Bioactive Proteins via Atypically Split Inteins.
Gharios R, Li A, Kopyeva I, Francis RM, DeForest CA
Bioconjug Chem. 2024 Jun 19;35(6):750-757. doi: 10.1021/acs.bioconjchem.4c00223. Epub 2024 May 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 241009 | pET-29_GSS-6xHis-SUMO-VidC-EGFP-FLAG | VidC-EGFP construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system. |
| 241010 | pET-29_GSS-6xHis-SUMO-VidC-mCherry-FLAG | VidC-mCherry construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system. |
| 241011 | pET-21_GSS-6xHis-SUMO-VidC-EGF-FLAG | VidC-EGF construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system. |
| 241012 | pET-21_GSS-6xHis-SUMO-VidC-bla-FLAG | VidC-bla construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system. |
| 241013 | pET-21_MBP-VidN | VidN construct that can be bacterially expressed, used to demonstrate intein splicing reaction. |