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Critical Analysis of Preprints and Inquiry-Based Lessons Improve the Synthetic Biology Learning Experience.

Nevot G, Guell M, Santos-Moreno J
ACS Synth Biol. 2025 Aug 15;14(8):2878-2884. doi: 10.1021/acssynbio.5c00014. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
238993pNA-YNode A carrying sgRNA-1 that can be used for the assembly of pEND-Y11
238994pNA-011Empty Node A that can be used for the assembly of pEND-11Y
238995pNA-ZNode A carrying sgRNA-2 that can be used for the assembly of pEND-Z11 or pEND-ZY
238996pNB-RepNode B carrying the sfGPF reporter that can be used for the assembly of pEND-11Y, pEND-Y11, pEND-Z11 or pEND-ZY
238997pNC-011Empty Node C that can be used for the assembly of pEND-Y11 and pEND-Z11
238998pNC-YNode C carrying sgRNA-1 that can be used for the assembly of pEND-11Y or pEND-ZY
238999pEND-11YFinal circuit with -Ara: OFF, +Ara: OFF behaviour
239000pEND-Y11Final circuit with -Ara: ON, +Ara: OFF behaviour
239001pEND-Z11Final circuit with -Ara: ON, +Ara: ON behaviour
239002pEND-ZYFinal circuit with -Ara: OFF, +Ara: ON behaviour

Antibodies from Article