De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy
Chen Y, Yserentant K, Hong K, Kuang Y, Bhowmick A, Charles-Orszag A, Lord SJ, Lu L, Hou K, Mann SI, Grimm JB, Lavis LD, Mullins RD, DeGrado WF, Huang B
bioRxiv 2025.06.24.661379
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239018 | pET29b-Rhobin2 | Bacterial overexpression of de novo Rhodamine binder 2 (Rhobin2) protein |
| 239019 | pET29b-Rhobin9 | Bacterial overexpression of de novo Rhodamine binder 9 (Rhobin2) protein |
| 239020 | pRhobin2-H2B-mNG3K-IRES-NLS-mTagBFP-2xNLS | Co-overexpression of Rhobin2-H2B-mNeonGreen3K fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker |
| 239021 | pRhobin9-H2B-mNG3K-IRES-NLS-mTagBFP-2xNLS | Co-overexpression of Rhobin9-H2B-mNeonGreen3K fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker |
| 239022 | pTOMM20-Rhobin2-IRES-NLS-mTagBFP-2xNLS | Co-overexpression of TOMM20-Rhobin2 fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker |
| 239023 | pTOMM20-Rhobin9-IRES-NLS-mTagBFP-2xNLS | Co-overexpression of TOMM20-Rhobin9 fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker |
| 239024 | pRhobin2-SEC61B-IRES-NLS-mTagBFP-2xNLS | Co-overexpression of Rhobin2-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker |
| 239025 | pRhobin9-SEC61B-IRES-NLS-mTagBFP-2xNLS | Co-overexpression of Rhobin9-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker |