High-performance genetically-encoded green and red fluorescent biosensors for pyruvate

Imai S, Hario S, Suerte C, Yamaguchi I, Sakoi K, Terai T, Takahashi-Yamashiro K, Campbell RE
bioRxiv 2025.04.17.649293 Article

Plasmids from Article

ID	Plasmid	Purpose
239049	pBAD-GreenPy1Highest	Bacterial expression of highest affinity green fluorescent pyruvate biosensor GreenPy1Highest
239050	pBAD-GreenPy1High	Bacterial expression of high affinity green fluorescent pyruvate biosensor GreenPy1High
239051	pBAD-GreenPy1Low	Bacterial expression of low affinity green fluorescent pyruvate biosensor GreenPy1Low
239052	pBAD-GreenPy1Lowest	Bacterial expression of lowest affinity green fluorescent pyruvate biosensor GreenPy1Lowest
239053	pBAD-dGreenPy1	Bacterial expression of green fluorescent pyruvate control biosensor dGreenPy1
239054	pBAD-ApplePy1Highest	Bacterial expression of highest affinity red fluorescent pyruvate biosensor ApplePy1Highest
239055	pBAD-ApplePy1High	Bacterial expression of high affinity red fluorescent pyruvate biosensor ApplePy1High
239056	pBAD-ApplePy1Low	Bacterial expression of low affinity red fluorescent pyruvate biosensor ApplePy1Low
239057	pBAD-ApplePy1Lowest	Bacterial expression of lowest affinity red fluorescent pyruvate biosensor ApplePy1Lowest
239058	pBAD-dApplePy1	Bacterial expression of red fluorescent pyruvate control biosensor dApplePy1
239059	pcDNA-GreenPy1Highest	Mammalian expression of highest affinity green fluorescent pyruvate biosensor GreenPy1Highest
239060	pcDNA-GreenPy1High	Mammalian expression of high affinity green fluorescent pyruvate biosensor GreenPy1High
239061	pcDNA-GreenPy1Low	Mammalian expression of low affinity green fluorescent pyruvate biosensor GreenPy1Low
239062	pcDNA-GreenPy1Lowest	Mammalian expression of lowest affinity green fluorescent pyruvate biosensor GreenPy1Lowest
239063	pcDNA-dGreenPy1	Mammalian expression of green fluorescent pyruvate control biosensor dGreenPy1
239064	pcDNA-ApplePy1Highest	Mammalian expression of highest affinity red fluorescent pyruvate biosensor ApplePy1Highest
239065	pcDNA-ApplePy1High	Mammalian expression of high affinity red fluorescent pyruvate biosensor ApplePy1High
239066	pcDNA-ApplePy1Low	Mammalian expression of low affinity red fluorescent pyruvate biosensor ApplePy1Low
239067	pcDNA-ApplePy1Lowest	Mammalian expression of lowest affinity red fluorescent pyruvate biosensor ApplePy1Lowest
239068	pcDNA-dApplePy1	Mammalian expression of red fluorescent pyruvate control biosensor dApplePy1
239069	pcDNA-mito-GreenPy1Highest	Mammalian expression of mitochondria-targeted highest affinity green fluorescent pyruvate biosensor GreenPy1Highest
239070	pcDNA-mito-GreenPy1High	Mammalian expression of mitochondria-targeted high affinity green fluorescent pyruvate biosensor GreenPy1High
239071	pcDNA-mito-GreenPy1Low	Mammalian expression of mitochondria-targeted low affinity green fluorescent pyruvate biosensor GreenPy1Low
239072	pcDNA-mito-GreenPy1Lowest	Mammalian expression of mitochondria-targeted lowest affinity green fluorescent pyruvate biosensor GreenPy1Lowest
239073	pcDNA-mito-dGreenPy1	Mammalian expression of mitochondria-targeted green fluorescent pyruvate control biosensor dGreenPy1
239074	pcDNA-mito-ApplePy1Highest	Mammalian expression of mitochondria-targeted highest affinity red fluorescent pyruvate biosensor ApplePy1Highest
239075	pcDNA-mito-ApplePy1High	Mammalian expression of mitochondria-targeted high affinity red fluorescent pyruvate biosensor ApplePy1High
239076	pcDNA-mito-ApplePy1Low	Mammalian expression of mitochondria-targeted low affinity red fluorescent pyruvate biosensor ApplePy1Low
239077	pcDNA-mito-ApplePy1Lowest	Mammalian expression of mitochondria-targeted lowest affinity red fluorescent pyruvate biosensor ApplePy1Lowest
239078	pcDNA-mito-dApplePy1	Mammalian expression of mitochondria-targeted red fluorescent pyruvate control biosensor dApplePy1