Amino acid positions near the active site determine the reduced activity of human ACOD1 compared to murine ACOD1.

Chen F, Yalcin I, Zhao M, Chen C, Blankenfeldt W, Pessler F, Bussow K
Sci Rep. 2023 Jun 26;13(1):10360. doi: 10.1038/s41598-023-37373-w. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
239117	pCAD130_hIrg1_M154I_pvp008	hACOD1 with M154I mutation in E.coli expression vector
239118	pCAD123_hIrg1_N152K,M154I_pvp008	hACOD1 with N152K and M154I mutations in E.coli expression vector
239119	pCAD183_gCAD_pET	Gorilla ACOD1 in E.coli expression vector
239120	pCAD131_hIrg1_M154I_pCMV6	hACOD1 with M154I mutation in mammalian expression vector
239122	pCAD182_gCAD_pCMV6-entry	Gorilla ACOD1 in mammalian expression vector