On-target toxicity limits the efficacy of CDK11 inhibition against cancers with 1p36 deletions

Julian LA, Crozier L, Lukow D, Mishra S, Swamy A, Hagenson RA, Sennhenn P, Sausville EL, Mendelson B, Chuaqui C, Qiao L, Vasudevan A, Lin K-T, Bhatia S, Bertomeu T, Chatr-aryamontri A, Zhang L, Rees MG, Ronan MM, Roth JA, Nottoli T, Bai S, Lakshmipathi J, Muthusamy V, Van Vranken JG, Gygi SP, Thompson SL, Smith JC, Anderson K, Shah S, Bindra RS, Akerman M, Spector DL, Krainer AR, Sheltzer JM
bioRxiv 2025.08.03.668359; doi: https://doi.org/10.1101/2025.08.03.668359 Article

Plasmids from Article

ID	Plasmid	Purpose
239211	pLV[Exp]-Bsd-CMV>hCDK11B	Expresses CDK11B in mammalian cell lines
239212	pLV[Exp]-Bsd-CMV>hCDK11A	Expresses CDK11A in mammalian cell lines
239213	pLV[Exp]-Bsd-CMV>hCDK11B G579S	Expresses CDK11B with G579S resistance mutation in mammalian cell lines
239214	pLV[Exp]-Bsd-CMV>hCDK11A G567S	Expresses CDK11A with G567S resistance mutation in mammalian cell lines
239215	pLV[Exp]-Bsd-CMV>hCDK11B-p58	Expresses CDK11B p58 isoform in mammalian cell lines
239216	pLV[Exp]-Bsd-CMV>hCDK11B-p58 G579S	Expresses CDK11B p58 isoform with G579S resistance mutation in mammalian cell lines
239217	pLV[Exp]-Bsd-CMV>hCDK11B D562A	Expresses CDK11B with D562A kinase inactivating mutation in mammalian cell lines
239218	pLV[Exp]-Bsd-CMV>hCDK11B D562A, G579S	Expresses CDK11B with D562A kinase inactivating mutation and G579S resistance mutation in mammalian cell lines
239219	pLV[Exp]-Neo-CMV>hCCNL2	Expresses CCNL2 in mammalian cell lines