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Hexokinase detachment from mitochondria drives the Warburg effect to support compartmentalized ATP production.

Huggler KS, Flickinger KM, Forsberg MH, Mellado Fritz CA, Chang GR, McGuire MF, Capitini CM, Cantor JR
Nat Metab. 2026 Jan 16. doi: 10.1038/s42255-025-01428-1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
239243pLJC2-HK2-3xFLAGHK2 lentiviral overexpression vector
239244pLJC2-HK2 (D209A, D657A)-3xFLAGHK2 lentiviral overexpression vector
239245pLJC2-TOM20-HK2-3xFLAGTOM20-HK2 lentiviral overexpression vector
239246pLJC5-HK2-3xFLAGHK2 lentiviral overexpression vector
239247pLJC5-HK1-3xFLAGHK1 lentiviral overexpression vector
239248pLJC5-3xMyc-eGFP-OMP253xMyc-eGFP-OMP25 lentiviral overexpression vector
239249pLJC5-3xHA-eGFP-OMP253xHA-eGFP-OMP25 lentiviral overexpression vector
239250pLJC6-HK2-3xFLAGHK2 lentiviral overexpression vector
239251pLJC6-HK2 (D209A, D657A)-3xFLAGHK2 lentiviral overexpression vector
239252pLJC6-HK1-3xFLAGHK1 lentiviral overexpression vector
239253pLJC6-HK1 (delta MBD)-3xFLAGHK1 lentiviral overexpression vector
239254pLJC6-HK2 (delta MBD)-3xFLAGHK2 lentiviral overexpression vector
239255pLJC6-MBD1-HK2-3xFLAGHK2 lentiviral overexpression vector
239256pLJC6-TOM20-HK2-3xFLAGTOM20-HK2 lentiviral overexpression vector
239257pLentiCRISPR-v1-sgHK2_14lentiviral vector expressing Cas9 and an sgRNA targeting HK2

Antibodies from Article