Programmable control of spatial transcriptome in live cells and neurons.

Han M, Fu ML, Zhu Y, Choi AA, Li E, Bezney J, Cai S, Miles L, Ma Y, Qi LS
Nature. 2025 Jul;643(8070):241-251. doi: 10.1038/s41586-025-09020-z. Epub 2025 May 21. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
239266	pSLQ10530	Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in the cytoplasm of cell lines
239267	pSLQ10531	Expresses NLS-dCas13b-ABI for CRISPR-TO perturbation in the nucleus of cell lines
239268	pSLQ10625	Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in primary mouse cortical neurons
239269	pSLQ10550	Expresses PYL1-MAVS* for recruiting RNA to the outer mitochondrial membrane via CRISPR-TO
239270	pSLQ10539	Expresses PYL1-DDX6 for recruiting RNA to the p-body via CRISPR-TO
239271	pSLQ10552	Expresses PYL1-G3BP1 for recruiting RNA to the stress granule via CRISPR-TO
239272	pSLQ14566	Expresses TRF1-PYL1 for recruiting RNA to the telomere via CRISPR-TO
239273	pSLQ14806	Expresses TRF1-PYL1 for recruiting RNA to the nuclear stress body via CRISPR-TO
239274	pSLQ10622	Expresses PYL1-KIFC1* for the retrograde transport of RNA along microtubules via CRISPR-TO
239275	pSLQ14256	Expresses Kif5a*-PYL1 for the anterograde transport of RNA along microtubules via CRISPR-TO in primary mouse cortical neurons
239276	pSLQ14901	Expresses gG1 for pertubing endogenous human GAPDH mRNA via CRISPR-TO
239277	pSLQ14902	Expresses gG2 for pertubing endogenous human GAPDH mRNA via CRISPR-TO