Identification of human pathways acting on nuclear non-coding RNAs using the Mirror forward genetic approach.
Che R, Panah M, Mirani B, Knowles K, Ostapovich A, Majumdar D, Chen X, DeSimone J, White W, Noonan M, Luo H, Alexandrov A
Nat Commun. 2025 May 22;16(1):4741. doi: 10.1038/s41467-025-59998-3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239295 | LentiCRISPR-B_sgRNA_EXOSC10 (pAVA3318) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC10 |
| 239296 | LentiCRISPR-B_sgRNA_C1D (pP18(T)_B9-AVA4070) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting C1D |
| 239297 | LentiCRISPR-B_sgRNA_DDX59 (pAVA3791) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DDX59 |
| 239298 | LentiCRISPR-B_sgRNA_DDX59 (pAVA3796) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DDX59 |
| 239299 | LentiCRISPR-B_sgRNA_DDX59 (pAVA3317) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DDX59 |
| 239300 | LentiCRISPR-B_sgRNA_DDX59 (pAVA3794) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DDX59 |
| 239301 | LentiCRISPR-B_sgRNA_EXOSC2 (pP18(T)_C3-AVA4067) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC2 |
| 239302 | LentiCRISPR-B_sgRNA_EXOSC4 (pP18(T)_C11-AVA4065) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC4 |
| 239303 | LentiCRISPR-B_sgRNA_EXOSC5 (pP18(T)_B12-AVA4068) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC5 |
| 239304 | LentiCRISPR-B_sgRNA_EXOSC6 (pP18(T)_B11-AVA4069) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC6 |
| 239305 | LentiCRISPR-B_sgRNA_EXOSC7 (pP18(T)_C4-AVA4066) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC7 |
| 239306 | LentiCRISPR-B_sgRNA_EXOSC8 (pP18(T)_B1-AVA4071) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC8 |
| 239307 | LentiCRISPR-B_sgRNA_EXOSC9 (pP18(T)_A7-AVA4072) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC9 |
| 239308 | LentiCRISPR-B_sgRNA_DIS3 (pP18(T)_A5-AVA4073) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DIS3 |
| 239310 | LentiCRISPR-B_sgRNA_D11 (pAVA3773) | Lentiviral vector expressing Cas9-P2A-Blasticidin and a non-targeting sgRNA as control(-) |
| 239311 | LentiCRISPR-B_sgRNA_POP1 (pAVA3497) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting POP1 |
| 239313 | LentiCRISPR-B_sgRNA_RPP38 (pAVA3523) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP38 |
| 239314 | LentiCRISPR-B_sgRNA_RPP29 (pAVA3545) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP29 |
| 239315 | LentiCRISPR-B_sgRNA_POP5 (pAVA3498) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting POP5 |
| 239316 | LentiCRISPR-B_sgRNA_RPP20 (pAVA3546) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP20 |
| 239317 | LentiCRISPR-B_sgRNA_RPP14 (pAVA3507) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP14 |
| 239318 | LentiCRISPR-B_sgRNA_RPP30 (pAVA3573) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP30 |
| 239319 | LentiCRISPR-B_sgRNA_RPP40 (pAVA3540) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP40 |
| 239320 | LentiCRISPR-B_sgRNA_RPP25 (pAVA3522) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP25 |
| 239321 | LentiCRISPR-B_sgRNA_RPP25L (pAVA3548) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP25L |
| 239322 | LentiCRISPR-B_2xsgRNAs_RBM7 (pAVA3250) | Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RBM7 |
| 239323 | LentiCRISPR-B_2xsgRNAs_SKIV2L2 (pAVA3251) | Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting SKIV2L2 |
| 239325 | LentiCRISPR-B_2xsgRNAs_ZCRB1 (pAVA3300) | Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNA stargeting ZCRB1 |
| 239326 | LentiCRISPR-B_2xsgRNAs_BRF2 (pAVA3258) | Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting BRF2 |
| 239327 | LentiCRISPR-B_2xsgRNAs_RPP21 (pAVA3259) | Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RPP21 |
| 239328 | LentiCRISPR-B_2xsgRNAs_RPPH1 (pAVA3586) | Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RPPH1 |
| 239329 | LentiCRISPR-B_2xsgRNAs_UPF3A/UPF3B (pAVA3129) | Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting UPF3A and UPF3B |
| 239345 | TCTN3 minigene (pAVA3939) | Expresses TCTN3 minigene (EFS-6xMyc-TCTN3-Ex10-I10-Ex11-copGFP-3xFLAG) |
| 239346 | pcDNA5/FRT/MALAT1[C8351G] (pAVA3169) | Expresses full-length, driven by its own promoter MALAT1 with stability-compromised ENE(C8351G) and an 11-nt insertion (cgctcgacgta). |
| 239347 | pcDNA5/FRT/MALAT1[WT] (pAVA3171) | Expresses full-length, driven by its own promoter MALAT1 with wild-type ENE and an 11-nt insertion (cgctcgacgta). |
| 239348 | Mirror RFP[ENE(WT)-mascRNA](pAVA2987) | Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of wild-type MALAT1 ENE-mascRNA reporter. |
| 239349 | Mirror GFP[ENE(C8351G)-mascRNA](pAVA3000) | Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of C8351G MALAT1 ENE-mascRNA reporter. |
| 239350 | Mirror RFP[ENE(C8351G)-mascRNA](pAVA2995) | Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of C8351G MALAT1 ENE-mascRNA reporter. |
| 239351 | Mirror GFP[ENE(WT)-mascRNA](pAVA2965) | Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of wild-type MALAT1 ENE-mascRNA reporter. |
| 239352 | Mirror GFP[ENE(C8351G)-mascRNA(mut 8356-8370)](pAVA3871) | Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of MALAT1 ENE(C8351G)-mascRNA(mut 8356-8370) reporter. |
| 239353 | Mirror GFP[ENE(WT)-mascRNA(mut 8356-8370)](pAVA3874) | Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of MALAT1 ENE(WT)-mascRNA(mut 8356-8370) reporter. |