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Identification of human pathways acting on nuclear non-coding RNAs using the Mirror forward genetic approach.

Che R, Panah M, Mirani B, Knowles K, Ostapovich A, Majumdar D, Chen X, DeSimone J, White W, Noonan M, Luo H, Alexandrov A
Nat Commun. 2025 May 22;16(1):4741. doi: 10.1038/s41467-025-59998-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
239295LentiCRISPR-B_sgRNA_EXOSC10 (pAVA3318)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC10
239296LentiCRISPR-B_sgRNA_C1D (pP18(T)_B9-AVA4070)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting C1D
239297LentiCRISPR-B_sgRNA_DDX59 (pAVA3791)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DDX59
239298LentiCRISPR-B_sgRNA_DDX59 (pAVA3796)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DDX59
239299LentiCRISPR-B_sgRNA_DDX59 (pAVA3317)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DDX59
239300LentiCRISPR-B_sgRNA_DDX59 (pAVA3794)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DDX59
239301LentiCRISPR-B_sgRNA_EXOSC2 (pP18(T)_C3-AVA4067)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC2
239302LentiCRISPR-B_sgRNA_EXOSC4 (pP18(T)_C11-AVA4065)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC4
239303LentiCRISPR-B_sgRNA_EXOSC5 (pP18(T)_B12-AVA4068)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC5
239304LentiCRISPR-B_sgRNA_EXOSC6 (pP18(T)_B11-AVA4069)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC6
239305LentiCRISPR-B_sgRNA_EXOSC7 (pP18(T)_C4-AVA4066)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC7
239306LentiCRISPR-B_sgRNA_EXOSC8 (pP18(T)_B1-AVA4071)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC8
239307LentiCRISPR-B_sgRNA_EXOSC9 (pP18(T)_A7-AVA4072)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC9
239308LentiCRISPR-B_sgRNA_DIS3 (pP18(T)_A5-AVA4073)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DIS3
239310LentiCRISPR-B_sgRNA_D11 (pAVA3773)Lentiviral vector expressing Cas9-P2A-Blasticidin and a non-targeting sgRNA as control(-)
239311LentiCRISPR-B_sgRNA_POP1 (pAVA3497)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting POP1
239313LentiCRISPR-B_sgRNA_RPP38 (pAVA3523)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP38
239314LentiCRISPR-B_sgRNA_RPP29 (pAVA3545)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP29
239315LentiCRISPR-B_sgRNA_POP5 (pAVA3498)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting POP5
239316LentiCRISPR-B_sgRNA_RPP20 (pAVA3546)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP20
239317LentiCRISPR-B_sgRNA_RPP14 (pAVA3507)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP14
239318LentiCRISPR-B_sgRNA_RPP30 (pAVA3573)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP30
239319LentiCRISPR-B_sgRNA_RPP40 (pAVA3540)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP40
239320LentiCRISPR-B_sgRNA_RPP25 (pAVA3522)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP25
239321LentiCRISPR-B_sgRNA_RPP25L (pAVA3548)Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP25L
239322LentiCRISPR-B_2xsgRNAs_RBM7 (pAVA3250)Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RBM7
239323LentiCRISPR-B_2xsgRNAs_SKIV2L2 (pAVA3251)Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting SKIV2L2
239325LentiCRISPR-B_2xsgRNAs_ZCRB1 (pAVA3300)Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNA stargeting ZCRB1
239326LentiCRISPR-B_2xsgRNAs_BRF2 (pAVA3258)Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting BRF2
239328LentiCRISPR-B_2xsgRNAs_RPPH1 (pAVA3586)Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RPPH1
239329LentiCRISPR-B_2xsgRNAs_UPF3A/UPF3B (pAVA3129)Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting UPF3A and UPF3B
239345TCTN3 minigene (pAVA3939)Expresses TCTN3 minigene (EFS-6xMyc-TCTN3-Ex10-I10-Ex11-copGFP-3xFLAG)
239346pcDNA5/FRT/MALAT1[C8351G] (pAVA3169)Expresses full-length, driven by its own promoter MALAT1 with stability-compromised ENE(C8351G) and an 11-nt insertion (cgctcgacgta).
239347pcDNA5/FRT/MALAT1[WT] (pAVA3171)Expresses full-length, driven by its own promoter MALAT1 with wild-type ENE and an 11-nt insertion (cgctcgacgta).

Antibodies from Article